Effective degradation of tetracycline by manganese peroxidase producing Bacillus velezensis strain Al-Dhabi 140 from Saudi Arabia using fibrous-bed reactor

Abstract

A tetracycline degrading bacterial strains was characterized from the municipal sludge and detected its ability to produce manganese peroxidase. The molecular weight of manganese peroxidase was determined as 46 kDa after Biogel P-100 gel filtration column chromatography purification. Maximum tetracycline degradation was observed with the manganese peroxidase from the strain Bacillus velezensis Al-Dhabi 140 and the optimum degradation process was studied. Optimization revealed the maximum removal efficacy was obtained as 87 mg/L at initial tetracycline concentration 143.75 mg/L, pH 6.94 and 8.04% inoculum. Consequently, fibrous bed reactor containing the culture of B. velezensis Al-Dhabi 140 in fibrous matrix was formed to transform tetracycline in synthetic wastewater. The transformed product of tetracycline from the fibrous bed reactor was evident by the activity of ligninolytic enzymes produced by B. velezensis Al-Dhabi 140 in reactor. The decreased level of antibacterial potency was obtained after 10 days. The zone of inhibition was 24 ± 1 mm after 1 day and it decreased as 9 ± 1 mm after 10 days. Based on the findings, fibrous bed B. velezensis Al-Dhabi 140 could be an efficient strain for tetracycline removal from artificial wastewater, even from natural wastewater.

Keywords: Biodegradation; Fibrous bed reactor; Manganese peroxidase; Tetracycline; Wastewater.