Virus isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for diagnostic and research purposes

doi:10.1016/j.pathol.2020.09.012

Review

. 2020 Dec;52(7):760-763.

doi: 10.1016/j.pathol.2020.09.012. Epub 2020 Oct 8.

Virus isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for diagnostic and research purposes

Sacha Stelzer-Braid¹, Gregory J Walker², Anupriya Aggarwal³, Sonia R Isaacs², Malinna Yeang⁴, Zin Naing⁵, Alberto Ospina Stella³, Stuart G Turville³, William D Rawlinson⁶

Affiliations

¹ Virology Research Laboratory, Serology and Virology Division (SAViD), Prince of Wales Hospital, Sydney, NSW, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia. Electronic address: s.stelzer-braid@unsw.edu.au.
² Virology Research Laboratory, Serology and Virology Division (SAViD), Prince of Wales Hospital, Sydney, NSW, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia.
³ Kirby Institute, University of New South Wales, Sydney, NSW, Australia.
⁴ Virology Research Laboratory, Serology and Virology Division (SAViD), Prince of Wales Hospital, Sydney, NSW, Australia; Serology and Virology Division (SAViD), NSW Health Pathology East, Department of Microbiology, Prince of Wales Hospital, Sydney, NSW, Australia.
⁵ Serology and Virology Division (SAViD), NSW Health Pathology East, Department of Microbiology, Prince of Wales Hospital, Sydney, NSW, Australia.
⁶ Virology Research Laboratory, Serology and Virology Division (SAViD), Prince of Wales Hospital, Sydney, NSW, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia; Serology and Virology Division (SAViD), NSW Health Pathology East, Department of Microbiology, Prince of Wales Hospital, Sydney, NSW, Australia.

PMID: 33131800
PMCID: PMC7543926
DOI: 10.1016/j.pathol.2020.09.012

Review

Virus isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for diagnostic and research purposes

Sacha Stelzer-Braid et al. Pathology. 2020 Dec.

. 2020 Dec;52(7):760-763.

doi: 10.1016/j.pathol.2020.09.012. Epub 2020 Oct 8.

Authors

Sacha Stelzer-Braid¹, Gregory J Walker², Anupriya Aggarwal³, Sonia R Isaacs², Malinna Yeang⁴, Zin Naing⁵, Alberto Ospina Stella³, Stuart G Turville³, William D Rawlinson⁶

Affiliations

¹ Virology Research Laboratory, Serology and Virology Division (SAViD), Prince of Wales Hospital, Sydney, NSW, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia. Electronic address: s.stelzer-braid@unsw.edu.au.
² Virology Research Laboratory, Serology and Virology Division (SAViD), Prince of Wales Hospital, Sydney, NSW, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia.
³ Kirby Institute, University of New South Wales, Sydney, NSW, Australia.
⁴ Virology Research Laboratory, Serology and Virology Division (SAViD), Prince of Wales Hospital, Sydney, NSW, Australia; Serology and Virology Division (SAViD), NSW Health Pathology East, Department of Microbiology, Prince of Wales Hospital, Sydney, NSW, Australia.
⁵ Serology and Virology Division (SAViD), NSW Health Pathology East, Department of Microbiology, Prince of Wales Hospital, Sydney, NSW, Australia.
⁶ Virology Research Laboratory, Serology and Virology Division (SAViD), Prince of Wales Hospital, Sydney, NSW, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia; Serology and Virology Division (SAViD), NSW Health Pathology East, Department of Microbiology, Prince of Wales Hospital, Sydney, NSW, Australia.

PMID: 33131800
PMCID: PMC7543926
DOI: 10.1016/j.pathol.2020.09.012

Abstract

Isolation of the new pandemic virus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for diagnostic and research purposes including assessment of novel therapeutics. Several primary and continuous cell lines are currently used, and new organoid and engineered cell lines are being developed for improved investigation and understanding of the human immune response to this virus. Here we review the growth of SARS-CoV-2 in reference standard cell lines, engineered cell lines and new developments in this field.

Keywords: SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; cell culture; respiratory virus; virus culture.

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References

1. Leland D.S., Ginocchio C.C. Role of cell culture for virus detection in the age of technology. Clin Microbiol Rev. 2007;20:49–78. - PMC - PubMed
1. La Scola B., Le Bideau M., Andreani J., et al. Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards. Eur J Clin Microbiol Infect Dis. 2020;39:1059–1061. - PMC - PubMed
1. Smith D.W. The challenges of establishing adequate capacity for SARS-CoV-2 testing. Med J Aust. 2020;212:457–458. - PMC - PubMed
1. Caly L., Druce J., Roberts J., et al. Isolation and rapid sharing of the 2019 novel coronavirus (SARS-CoV-2) from the first patient diagnosed with COVID-19 in Australia. Med J Aust. 2020;212:459–462. - PMC - PubMed
1. Harcourt J., Tamin A., Lu X., et al. Severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States. Emerg Infect Dis. 2020;26:1266–1273. - PMC - PubMed

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[1] Leland D.S., Ginocchio C.C. Role of cell culture for virus detection in the age of technology. Clin Microbiol Rev. 2007;20:49–78. - PMC - PubMed

[2] Leland D.S., Ginocchio C.C. Role of cell culture for virus detection in the age of technology. Clin Microbiol Rev. 2007;20:49–78. - PMC - PubMed

[3] La Scola B., Le Bideau M., Andreani J., et al. Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards. Eur J Clin Microbiol Infect Dis. 2020;39:1059–1061. - PMC - PubMed

[4] La Scola B., Le Bideau M., Andreani J., et al. Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards. Eur J Clin Microbiol Infect Dis. 2020;39:1059–1061. - PMC - PubMed

[5] Smith D.W. The challenges of establishing adequate capacity for SARS-CoV-2 testing. Med J Aust. 2020;212:457–458. - PMC - PubMed

[6] Smith D.W. The challenges of establishing adequate capacity for SARS-CoV-2 testing. Med J Aust. 2020;212:457–458. - PMC - PubMed

[7] Caly L., Druce J., Roberts J., et al. Isolation and rapid sharing of the 2019 novel coronavirus (SARS-CoV-2) from the first patient diagnosed with COVID-19 in Australia. Med J Aust. 2020;212:459–462. - PMC - PubMed

[8] Caly L., Druce J., Roberts J., et al. Isolation and rapid sharing of the 2019 novel coronavirus (SARS-CoV-2) from the first patient diagnosed with COVID-19 in Australia. Med J Aust. 2020;212:459–462. - PMC - PubMed

[9] Harcourt J., Tamin A., Lu X., et al. Severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States. Emerg Infect Dis. 2020;26:1266–1273. - PMC - PubMed

[10] Harcourt J., Tamin A., Lu X., et al. Severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States. Emerg Infect Dis. 2020;26:1266–1273. - PMC - PubMed

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Virus isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for diagnostic and research purposes

Affiliations

Virus isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for diagnostic and research purposes

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