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Review
. 2020 Dec;52(7):760-763.
doi: 10.1016/j.pathol.2020.09.012. Epub 2020 Oct 8.

Virus isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for diagnostic and research purposes

Sacha Stelzer-Braid  1 , Gregory J Walker  2 , Anupriya Aggarwal  3 , Sonia R Isaacs  2 , Malinna Yeang  4 , Zin Naing  5 , Alberto Ospina Stella  3 , Stuart G Turville  3 , William D Rawlinson  6
Affiliations
Review

Virus isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for diagnostic and research purposes

Sacha Stelzer-Braid et al. Pathology. 2020 Dec.

Abstract

Isolation of the new pandemic virus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for diagnostic and research purposes including assessment of novel therapeutics. Several primary and continuous cell lines are currently used, and new organoid and engineered cell lines are being developed for improved investigation and understanding of the human immune response to this virus. Here we review the growth of SARS-CoV-2 in reference standard cell lines, engineered cell lines and new developments in this field.

Keywords: SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; cell culture; respiratory virus; virus culture.

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