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. 2020 Summer;11(3):249-256.
doi: 10.30466/vrf.2019.97340.2321. Epub 2020 Sep 15.

The effect of intracerebroventricular administration of neuropeptide Y on reproductive axis function in the male Wistar rats: Involvement of hypothalamic KiSS1/GPR54 system

Vahid Azizi  1 , Shahrbanoo Oryan  1 , Homayuon Khazali  2
Affiliations

The effect of intracerebroventricular administration of neuropeptide Y on reproductive axis function in the male Wistar rats: Involvement of hypothalamic KiSS1/GPR54 system

Vahid Azizi et al. Vet Res Forum. 2020 Summer.

Abstract

Several studies have shown that neuropeptide Y (NPY) is considered to be one of the key regulators of the hypothalamic-pituitary-gonadal axis in the mammals. Also, kisspeptin is a powerful upstream regulator of gonadotropin-releasing hormone neurons in the hypothalamus. The present study aims to investigate the effects of the intracerebroventricular (ICV) injection of NPY and BIBP3226 (NPY receptor antagonist) on the reproductive axis (either hormonal or behavioral) of the male rats. Furthermore, to see whether NPY signals can be relayed through the pathway of KiSS1/GPR54, the gene expression of these peptides in the arcuate nucleus was measured. The ICV injection of NPY decreased the latencies and increased the frequencies of sexual parameters of the male rats in a significant way. Results obtained from LH and testosterone measurement showed that NPY had a significant increase in comparison with the control group. In this line, BIBP3226 antagonized the stimulative effects of NPY. Furthermore, data from real-time quantitative PCR showed that injection of NPY significantly increased the gene expression of KiSS1 and GPR54, while treatment with BIBP3226 controlled the stimulative effects of NPY on gene expression of KiSS1 and GPR54. Summing up, NPY can exert its impacts on the reproductive axis, this occurs at least partly through affecting KiSS1/GPR54 system.

Keywords: GPR54; Gene expression; Kisspeptin; Neuropeptide Y; Sexual behavior.

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Figures

Fig. 1
Fig. 1
A, B, and C) Serum LH concentration; and D, E, and F) serum testosterone levels at different times by administration of NPY, BIBP3226 and co-administration of NPY and BIBP3226 via third ventricle (n = 6 in each group). **, p < 0.01; ***, p < 0.001 compared to control (vehicle) at corresponding time (30 and 60 min); +, p < 0.05; ++, p < 0.01; +++, p < 0.001 compared to the corresponding time (0 min).
Fig. 2
Fig. 2
A, B, and C) Relative mRNA expression (arbitrary units) of KiSS1; and D, E, and F) Relative mRNA expression of GPR54 in the rat arcuate nucleus after administration of NPY, BIBP3226 and co-administration of NPY and BIBP3226 compared to the control group (n = 6 in each group). ***p < 0.001
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