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. 2020 Oct 27:6:75.
doi: 10.1038/s41421-020-00224-3. eCollection 2020.

Sensitivity and specificity of SARS-CoV-2 S1 subunit in COVID-19 serology assays

Ying Tian #  1 , Chaoyang Lian #  1 , Yingying Chen #  1 , Dong Wei  2 , Xinxin Zhang  2 , Yun Ling  3 , Ying Wang  1 , Leng-Siew Yeap  1
Affiliations

Sensitivity and specificity of SARS-CoV-2 S1 subunit in COVID-19 serology assays

Ying Tian et al. Cell Discov. .
No abstract available

Keywords: Immunology; Molecular biology.

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Conflict of interest statement

Conflict of interestThe authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1. ELISA assays to detect anti-SARS-CoV-2 antibodies in human coronaviruses infected individuals.
a Schematic showing SARS-CoV-2 S trimer, S1 and RBD. b ELISA measurement of plasma reactivity to RBD, S1, and S trimer. Y-axis is OD 450 nm normalized to the mean absorbance of CR3022 in each plate. X-axis is log reciprocal plasma dilution (upper row). Normalized area under the curve (AUC) of 18 controls (gray) and 45 patients (35 patients infected with SARS-CoV-2, red; 4 patients infected with HCoV-OC43, blue; 5 patients infected with HCoV-HKU1, green; and 1 patient infected with HCoV-229E, magenta) for IgG binding to SARS-CoV-2 S1, S trimer, and RBD (lower row). Data were expressed as mean. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. c Correlation between AUC of anti-SARS-CoV-2 RBD and AUC of anti-SARS-CoV-2 S1 (left) or anti-SARS-CoV-2 S trimer (right). d Correlation between AUC of anti-SARS-CoV-2 RBD and AUC of anti-SARS-CoV RBD. e Demographic details and normalized AUC of all samples for IgG binding to β-CoV (SARS-CoV-2 S1, S trimer, RBD, SARS-CoV RBD, MERS-CoV S1, and HCoV-HKU1 S1) and α-CoV (HCoV-229E S1 and HCoV-NL63 S1) presented in bar chart.
See this image and copyright information in PMC

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