Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jun 24;10(6):555-564.
doi: 10.1016/j.jtcme.2019.06.005. eCollection 2020 Nov.

Antidiabetic activity of corn steep liquor extract of Curculigo pilosa and its solvent fractions in streptozotocin-induced diabetic rats

Kayode Olayele Karigidi  1   2 , Charles Ojo Olaiya  1
Affiliations

Antidiabetic activity of corn steep liquor extract of Curculigo pilosa and its solvent fractions in streptozotocin-induced diabetic rats

Kayode Olayele Karigidi et al. J Tradit Complement Med. .

Abstract

The rhizome of Curculigo pilosa (CP) prepared with Corn steep liquor (CSL), is traditionally used in the treatment of diabetes mellitus. In this study, antidiabetic activity of the CSL extract and its fractions (butanol and methanol) were evaluated in streptozotocin-induced diabetic rats. Diabetes mellitus was induced by single intraperitoneal administration of streptozotocin (50 mg/kg) and diabetic rats were treated with 300 mg/kg bodyweight of the extract(s) for 28 days. Antidiabetic effect was monitored by plasma blood glucose, oral glucose tolerances test (OGGT), body weight and heamatological indices. Also serum urea, creatinine, cholesterol, high density lipoprotein, bilirubin, alkaline phosphatase, aspartate and alanine transaminases were evaluated. The levels of hepatic glutathione, catalase, superoxide dismutase, lipid peroxidation, nitric oxide and hydrogen peroxide were assessed; also histopathology of the hepatic tissues was examined. Oral administration of the extract resulted in significant (p < 0.05) reduction of plasma blood glucose (29.32% crude extract and 22.96% butanol fraction) and also increased body weight (20.61% crude extract, 13.44% butanol fraction and 6.23% methanol fraction) of diabetic rats. The heamotogical indices, plasma parameters and hepatic oxidative stress in diabetic rats were returned to near normalcy following treatment with the extract(s). The GC-MS analysis of the extract revealed the presence of stilbene, a proven antidiabetic agent, which might be responsible for the antidiabetic activity. The results obtained suggest that the CSL extract of CP could be used in management of diabetes mellitus thus providing scientific validation of its use as an antidiabetic agent.

Keywords: Corn steep liquor; Curculigo pilosa; Streptozotocin; antidiabetic.

PubMed Disclaimer

Figures

Image 1
Graphical abstract
Fig. 1
Fig. 1
Effect of CSL extract of Curculigo pilosa and its solvent fractions on OGTT (mmol/l) in streptozotocin-induced diabetic rats. a p < 0.05 when experimental groups were compared with control group, b p < 0.05 when experimental groups were compared with diabetic control group.
Fig. 2
Fig. 2
Effect of CSL extract of Curculigo pilosa and its solvent fractions on kidney damage biomarkers (a) Urea (b) Creatinine in streptozotocin-induced diabetic rats. a p < 0.05 when experimental groups were compared with control group, b p < 0.05 when experimental groups were compared with diabetic control group.
Fig. 3
Fig. 3
Effect of CSL extracts of Curculigo pilosa and its solvent fractions on liver damage biomarkers (a) AST (b) ALP (c) TB (d) ALT in streptozotocin-induced diabetic rats. a p < 0.05 when experimental groups were compared with control group, b p < 0.05 when experimental groups were compared with diabetic control group.
Fig. 4
Fig. 4
Effect of CSL extracts of Curculigo pilosa and its solvent fractions on lipid profiles (a) Cholesterol (b) HDL-Cholesterol (c) Atherogenic index (d) Cardiac risk ratio in streptozotocin-induced diabetic rats. a p < 0.05 when experimental groups were compared with control group, b p < 0.05 when experimental groups were compared with diabetic control group.
Fig. 5
Fig. 5
Effect of CSL extracts of Curculigo pilosa and its solvent fractions on hepatic (a) GSH (b) LPO (c) NO (d) H2O2 (e) Catalase (f) SOD in streptozotocin-induced diabetic rats. a p < 0.05 when experimental groups were compared with control group, b p < 0.05 when experimental groups were compared with diabetic control group.
Fig. 6
Fig. 6
Photomicrographs ( × 100) of hepatic tissue of control and experimental groups of rat (stained with H&E). A: Control, B: Dcontrol, C: CRD, D: BUTH, E: METH, F: GLBC.
Fig. 7
Fig. 7
Chromatogram of Corn steep liquor extract of Curculigo pilosa by GC-MS.
See this image and copyright information in PMC

References

    1. Oyedemi S.O., Adewusi E.A., Aiyegoro O.A., Akinpelu D.A. Antidiabetic and haematological effect of aqueous extract of stem bark of Afzelia Africana (Smith) on streptozotocin-induced diabetic Wistar rats. Asian Pac J Trop Biomed. 2011;1(5):353–358. - PMC - PubMed
    1. International Diabetes Federation the IDF Diabetes Atlas. eighth ed. 2017.
    1. Snehal N.M., Jayesh B.D., Sangita B.K., Archana R.J. Attenuation of diabetic nephropathy in streptozotocin-induced diabetic rats by Punica granatum Linn. leaves extract. J Tradit Complemen Med. 2017;7:273–280. - PMC - PubMed
    1. International Diabetes Federation the IDF Diabetes Atlas. seventh ed. 2015.
    1. Zurina H., Mun F.Y., Mariam A., Ahmad P.M.Y. Antidiabetic properties and mechanism of action of Gynura procumbens water extract in streptozotocin-induced diabetic rats. Molecules. 2010;15:9008–9023. - PMC - PubMed

LinkOut - more resources