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. 2020 Jul 25;42(5):370-375.
doi: 10.1016/j.pld.2020.07.003. eCollection 2020 Oct.

A hybrid sterile locus leads to the linkage drag of interspecific hybrid progenies

Mianmian Wang  1   2 , Jun Yang  1   2 , Jinpeng Wan  1   3 , Dayun Tao  4 , Jiawu Zhou  4 , Diqiu Yu  1   3 , Peng Xu  1   3
Affiliations

A hybrid sterile locus leads to the linkage drag of interspecific hybrid progenies

Mianmian Wang et al. Plant Divers. .

Abstract

Interspecific hybridization plays an important role in rice breeding by broadening access to desirable traits such as disease resistance and improving yields. However, interspecific hybridization is often hindered by hybrid sterility, linkage drag, and distorted segregation. To mine for favorable genes from Oryza glaberrima, we cultivated a series of BC4 introgression lines (ILs) of O. glaberrima in the japonica rice variety background (Dianjingyou 1) in which the IL-2769 (BC4F10) showed longer sterile lemmas, wider grains and spreading panicles compared with its receptor parent, suggesting that linkage drag may have occurred. Based on the BC5F2 population, a hybrid sterility locus, S20, a long sterile lemma locus, G1-g, and a new grain width quantitative trait locus (QTL), qGW7, were mapped in the linkage region about 15 centimorgan (cM) from the end of the short arm of chromosome 7. The hybrid sterility locus S20 from O. glaberrima eliminated male gametes of Oryza sativa, and male gametes carrying the alleles of O. sativa in the heterozygotes were aborted completely. In addition, the homozygotes presented a genotype of O. glaberrima, and homozygous O. sativa were not produced. Surprisingly, the linked traits G1-g and qGW7 showed similar segregation distortion. These results indicate that S20 was responsible for the linkage drag. As a large number of detected hybrid sterility loci are widely distributed on rice chromosomes, we suggest that hybrid sterility loci are the critical factors for the linkage drag in interspecific and subspecific hybridization of rice.

Keywords: Hybrid sterility; Interspecific hybridization; Linkage drag; Segregation distortion.

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Figures

Fig. 1
Fig. 1
Phenotypes of BC5F1, DJY1 and IL-2769. (a–c) Pollen fertility of DJY1, BC5F1 hybrid and IL-2769, respectively; (d) Grain comparison of DJY1 and IL-2769 (n = 15); (e) Panicle type of DJY1, BC5F1 hybrid and IL-2769; (f) Plant architecture of two parents.
Fig. 2
Fig. 2
Distribution of traits in BC5F2 population.
Fig. 3
Fig. 3
The positions of linkage traits in the introgression region at the end of the short arm of chromosome 7.
See this image and copyright information in PMC

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