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. 2020 Oct;10(6):e212.
doi: 10.1002/ctm2.212.

Instantaneous "catch-and-kill" inactivation of SARS-CoV-2 by nitride ceramics

Giuseppe Pezzotti  1   2 , Eriko Ohgitani  2 , Masaharu Shin-Ya  2 , Tetsuya Adachi  3 , Elia Marin  1   3 , Francesco Boschetto  1   3 , Wenliang Zhu  1 , Osam Mazda  2
Affiliations

Instantaneous "catch-and-kill" inactivation of SARS-CoV-2 by nitride ceramics

Giuseppe Pezzotti et al. Clin Transl Med. 2020 Oct.
No abstract available

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Figures

FIGURE 1
FIGURE 1
(A) TCID50/50 μL and % reduction plots by TCID50 assay (based on the Reed‐Muench method). (B) RT‐PCR tests to evaluate viral RNA using two sets of N gene primers; a comparison is given using evaluations of supernatants and powders with viral RNA from virions simply suspended in water. (C) Fluorescence micrographs inoculated VeroE6/TMPRSS2 cells after staining: red, green, and blue stains visualize viral protein, F‐actin, and cell nuclei, respectively. (D) Quantification of fluorescence microscopy data given as % infected cells on total cells, namely, the percent fraction of red‐stained cells with respect to the total number of blue‐stained nuclei, and the percent fraction of viable cells on total cells, namely, the percent fraction of green‐stained cells with respect to the total number of blue‐stained nuclei. Labels in inset specify statistics (unpaired two‐tailed Student's test with n = 3)
FIGURE 2
FIGURE 2
The “catch and kill” mechanism. Central panel: Draft of the electrochemical interaction between Si3N4 surface and SARS‐CoV‐2 virions (envelope and membrane proteins are electrostatically attracted at the negatively charged Si3N4 surface while protonated amines, which resemble cell lysine N‐terminal receptors, link with the spike protein and lock the virions; once the virion is “caught” and locked on the Si3N4 surface, eluted NH3 gas freely penetrates envelope proteins and “kills” it). Left panel: Draft of electrochemical “binding competitive” interactions between protonated amine groups on the surface of Si3N4 and lysine N‐terminals in cells. Right panel: RNA cleavage by ammonia species occurs in three successive steps including the deprotonation of backbone 2′‐hydroxyls, the formation of a transient pentaphosphate group, and the final RNA cleavage by alkaline transesterification
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