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. 2020 Oct 29;10(11):1986.
doi: 10.3390/ani10111986.

Influence of Season and Liquid Storage at 16 °C on Beni Arouss Bucks' Semen Quality

Sara El Kadili  1   2 , Nathalie Kirschvink  1 , Marianne Raes  1 , Jean Loup Bister  1 , Bouchaib Archa  2 , Ahmed Douaik  3 , Mouad Chentouf  4
Affiliations

Influence of Season and Liquid Storage at 16 °C on Beni Arouss Bucks' Semen Quality

Sara El Kadili et al. Animals (Basel). .

Abstract

The study aimed at determining the effect of storage and season on fresh semen of Beni Arouss goats. Ejaculates were collected at monthly intervals from seven mature bucks and were extended at a final concentration of 800 × 106 spermatozoa. ml-1 and stored at 16 °C for 24 h. Semen motility, viability and normal morphology were assessed at 0, 4, 8 and 24 h after collection. Motility and normal morphology parameters were recorded using computer-assisted sperm analysis (CASA) and viability was analyzed using eosin-nigrosin staining. As expected, motility, viability and normal morphology parameters showed a significant reduction within 24 h of storage and during all seasons (p < 0.05). However, semen collected in summer maintained a better quality after 24 h of storage at 16 °C than semen collected during the other periods (p < 0.05). In conclusion, the storage ability of Beni Arouss bucks' semen stored at 16 °C was significantly higher during the summer.

Keywords: Beni Arouss buck; liquid storage; motility; normal morphology; season; viability.

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Conflict of interest statement

The authors declare that there is no conflict of interest that would prejudice the impartiality in conducting the experiment and publishing the manuscript.

Figures

Figure 1
Figure 1
Mean (±SD) of Beni Arouss bucks’ sperm total motility (A), progressive motility (B) and rapidly progressive motile spermatozoa (C) after 0, 4, 8 and 24 h of storage at 16°C and during the four seasons of the year. A, B, C, D: different capital letters indicate a significant effect of storage duration within the same season (p < 0.05). a, b, c: different lower-case letters indicate a significant effect of season at the same storage duration (p < 0.05).
Figure 2
Figure 2
Mean (±SD) of Beni Arouss bucks’ sperm velocities: curvilinear velocity (A), straight-line velocity (B) and (C) average path velocity after 0, 4, 8 and 24 h of storage at 16 °C and during the four seasons of the year. A, B, C, D: different capital letters indicate a significant effect of storage duration within the same season (p < 0.05). a, b, c: different lower-case letters indicate a significant effect of season at the same storage duration (p < 0.05).
Figure 3
Figure 3
Mean (±SD) of Beni Arouss bucks’ sperm viability after 0, 4, 8 and 24 h of storage at 16 °C and during the four seasons of the year. A, B, C, D: different capital letters indicate a significant effect of storage duration within the same season (p < 0.05). a, b, c: different lower-case letters indicate a significant effect of season at the same storage duration (p < 0.05).
Figure 4
Figure 4
Mean (±SD) of Beni Arouss bucks’ sperm normal morphology after 0, 4, 8 and 24 h of storage at 16 °C and during the four seasons of the year. A, B, C, D: different capital letters indicate a significant effect of storage duration within the same season (p < 0.05). a, b, c: different lower-case letters indicate a significant effect of season at the same storage duration (p < 0.05).
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