Pseudoalteromonas caenipelagi sp. nov., isolated from a tidal flat

Abstract

A Gram-stain-negative, aerobic, non-spore-forming, motile by single polar flagellum and ovoid or rod-shaped bacterial strain, designated JBTF-M23^T, was isolated from tidal flat sediment collected from the Yellow Sea, Republic of Korea. Neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain JBTF-M23^T fell within the clade comprising the type strains of Pseudoalteromonas species, clustering with the type strains of P. byunsanensis and P. amylolytica. Strain JBTF-M23^T exhibited the highest 16S rRNA gene sequence similarity value (98.6 %) to the type strain of P. rubra and sequence similarities of 98.3 and 97.7 % to the type strains of P. byunsanensis and P. amylolytica, respectively. The DNA G+C content of strain JBTF-M23^T from genomic sequence data was 41.98 %. The ANI and dDDH values between strain JBTF-M23^T and the type strains of P. rubra, P. byunsanensis and P. amylolytica were 71.3-76.6 and 19.4-19.9 %, respectively. Strain JBTF-M23^T contained Q-8 as the predominant ubiquinone and C_{16 : 1} ω7c and/or C_{16 : 1} ω6c, C_{16 : 0} and C_{18 : 1} ω7c as the major fatty acids. The major polar lipids of strain JBTF-M23^T were phosphatidylethanolamine and one unidentified aminolipid. Distinguished phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain JBTF-M23^T is separated from recognized Pseudoalteromonas species. On the basis of the data presented, strain JBTF-M23^Tis considered to represent a novel species of the genus Pseudoalteromonas, for which the name Pseudoalteromonas caenipelagi sp. nov. is proposed. The type strain is JBTF-M23^T(=KACC 19900^T=NBRC 113647^T).

Keywords: Pseudoalteromonas caenipelagi; genome; novel species; polyohasic taxonomy; tidal flat.