Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2020 Oct 31;8(11):464.
doi: 10.3390/biomedicines8110464.

Bioengineered Skin Intended as In Vitro Model for Pharmacosmetics, Skin Disease Study and Environmental Skin Impact Analysis

Raquel Sanabria-de la Torre  1   2   3 , Ana Fernández-González  1   2   3 , María I Quiñones-Vico  1   2   3 , Trinidad Montero-Vilchez  2   4 , Salvador Arias-Santiago  1   2   3   4   5
Affiliations
Review

Bioengineered Skin Intended as In Vitro Model for Pharmacosmetics, Skin Disease Study and Environmental Skin Impact Analysis

Raquel Sanabria-de la Torre et al. Biomedicines. .

Abstract

This review aims to be an update of Bioengineered Artificial Skin Substitutes (BASS) applications. At the first moment, they were created as an attempt to replace native skin grafts transplantation. Nowadays, these in vitro models have been increasing and widening their application areas, becoming important tools for research. This study is focus on the ability to design in vitro BASS which have been demonstrated to be appropriate to develop new products in the cosmetic and pharmacology industry. Allowing to go deeper into the skin disease research, and to analyze the effects provoked by environmental stressful agents. The importance of BASS to replace animal experimentation is also highlighted. Furthermore, the BASS validation parameters approved by the OECD (Organisation for Economic Co-operation and Development) are also analyzed. This report presents an overview of the skin models applicable to skin research along with their design methods. Finally, the potential and limitations of the currently available BASS to supply the demands for disease modeling and pharmaceutical screening are discussed.

Keywords: animal experimentation; atopic dermatitis; bioengineered artificial skin substitutes; fibroblasts; keratinocytes; product testing; psoriasis; regenerative medicine; tissue engineering; transepidermal water loss.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Diagram of the different applications of BASS: clinical applications, modeling physiological and pathological process and drugs discovery. Created with BioRender.com.
Figure 2
Figure 2
Diagram of the applications of BASS in research: pharmacosmetic development, environmental impacts and skin disease models. Created with BioRender.com.
See this image and copyright information in PMC

References

    1. Chandra P.K., Soker S., Atala A. Tissue engineering: Current status and future perspectives. In: Lanza R., Langer R., Vacanti J., Atala A., editors. Principles of Tissue Engineering. Volume 7. Academic Press; Winston-Salem, NC, USA: 2020. p. 1800457.
    1. Lee K.H. Tissue-engineered Human Living Skin Substitutes: Development and Clinical Application. Yonsei Med. J. 2000;41:774–779. doi: 10.3349/ymj.2000.41.6.774. - DOI - PubMed
    1. Burger S.R. Current regulatory issues in cell and tissue therapy. Cytotherapy. 2003;5:289–298. doi: 10.1080/14653240310002324. - DOI - PubMed
    1. Fernández-González A., Lizana-Moreno A., Ruiz-Garcia A., Guerrero-Calvo J., Espinosa O., Fernández N., Ordonez-Luque A., Sierra-Sanchez A., Arias-Santiago S. Optimization of an autologous human skin model under GMP guidelines. Cytotherapy. 2017;19:S232–S233. doi: 10.1016/j.jcyt.2017.02.338. - DOI
    1. Carriel V., Garzón I., Jiménez J.M., Oliveira A.C.X., Arias-Santiago S., Campos A., Sánchez-Quevedo M.C., Alaminos M. Epithelial and stromal developmental patterns in a novel substitute of the human skin generated with fibrin-agarose biomaterials. Cells Tissues Organs. 2012;196:1–12. doi: 10.1159/000330682. - DOI - PubMed