Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2021 Feb;118(2):542-554.
doi: 10.1002/bit.27620. Epub 2020 Nov 17.

A review of manufacturing capabilities of cell spheroid generation technologies and future development

Dan Liu  1 , Sixun Chen  1 , May Win Naing  1   2
Affiliations
Review

A review of manufacturing capabilities of cell spheroid generation technologies and future development

Dan Liu et al. Biotechnol Bioeng. 2021 Feb.

Abstract

Spheroid culture provides cells with a three-dimensional environment that can better mimic physiological conditions compared to monolayer culture. Technologies involved in the generation of cell spheroids are continuously being innovated to produce spheroids with enhanced properties. In this paper, we review the manufacturing capabilities of current cell spheroid generation technologies. We propose that spheroid generation technologies should enable tight and robust process controls to produce spheroids of consistent and repeatable quality. Future technology development for the generation of cell spheroids should look into improvement in process control, standardization, scalability and monitoring, in addition to advanced methods of spheroid transfer and characterization.

Keywords: 3D cell culture; cell spheroid; spheroid generation; spheroid manufacturing.

PubMed Disclaimer

References

REFERENCES

    1. Abe-Fukasawa, N., Otsuka, K., Aihara, A., Itasaki, N., & Nishino, T. (2018). Novel 3D liquid cell culture method for anchorage-independent cell growth, cell Imaging and automated drug screening. Scientific Reports, 8(1), 3627.
    1. Allen, L. M., Matyas, J., Ungrin, M., Hart, D. A., & Sen, A. (2019). Serum-free culture of human mesenchymal stem cell aggregates in suspension bioreactors for tissue engineering applications. Stem Cells International, 2019, 1-18.
    1. Anada, T., Sato, T., Kamoya, T., Shiwaku, Y., Tsuchiya, K., Takano-Yamamoto, T., Sasaki, K., & Suzuki, O. (2016). Evaluation of bioactivity of octacalcium phosphate using osteoblastic cell aggregates on a spheroid culture device. Regenerative Therapy, 3, 58-62.
    1. Baillargeon, P., Shumate, J., Hou, S. R., Fernandez-Vega, V., Marques, N., Souza, G., Seldin, J., Spicer, T. P., & Scampavia, L. (2019). Automating a magnetic 3D spheroid model technology for high-throughput screening. Slas Technology, 24(4), 420-428.
    1. Bayat, N., Ebrahimi-Barough, S., Norouzi-Javidan, A., Saberi, H., Ardakan, M. M. M., Ai, A., Soleimannejad, M., & Ai, J. (2018). Anti-inflammatory effects of atorvastatin by suppressing TRAF3IP2 and IL-17RA in human glioblastoma spheroids cultured in a three-dimensional model: Possible relevance to glioblastoma treatment. Molecular Neurobiology, 55(3), 2102-2110.

Publication types

LinkOut - more resources