Rhizosphere iron and manganese-oxidizing bacteria stimulate root iron plaque formation and regulate Cd uptake of rice plants (Oryza sativa L.)

Abstract

Iron plaque is the amorphous and/or crystalline layer of Fe and Mn (hydr)oxides formed on the root surface of wetland plants. It could adsorb and co-precipitate metal(loid)s at the rhizosphere, thus modulating the uptake and accumulation of metal elements in plants. In this study, the Fe(II)/Mn(II)-oxidizing bacteria Burkholderia sp. D416 (D416) and Pseudomonas sp. YGL (YGL) were isolated from Cd-contaminated rice field, both hydroponic experiment and pot experiment were performed to assess the impact of bacterial inoculation on iron plaque formation, elemental content of the plaque, plant dry mass, antioxidant enzyme activity and Cd content in rice plants. The results revealed that inoculation with D416, YGL, and D416+YGL stimulated iron plaque formation on the root surface of the hydroponic rice. The content of C, N, O, Na, Mg, Al, Si, P, S, Cl, K, Fe and Ca in the root plaque were affected by the bacterial inoculation and varied among different plant growth stages. The pot experiment indicated that inoculation with D416 increased the root dry biomass by 58.89%, and the combined inoculation of D416 and YGL increased the dry biomass of root, shoot and grain by 16.89%, 21.66% and 23.26%, respectively. Importantly, YGL inoculation decreased the Cd translocation from root to shoot and from glume to brown rice grain by 50.00% and 50.27%, respectively, and the Cd content in shoot and brown rice grain were decreased by 20.00% and 34.48%, respectively. Taken together, the elemental content of the iron plaque and Cd content in rice plants varied among different plant growth stages and when plants were inoculated with different bacterial strains. YGL dramatically reduced the Cd content in brown rice grain, thus it could potentially be used to reduce Cd content in rice crop grown in Cd-contaminated soils.

Keywords: Cadmium; Heavy metal accumulation; Iron and manganese-oxidizing bacteria; Iron oxidation; Iron plaque.