Toxicity evaluation of bulk and nanosheet MoS2 catalysts using battery bioassays

Abstract

Herein, the main aim is to study the influence of the materials' structural properties on their ecotoxicological properties. The acute toxicity of the bulk (molybdenum disulfide) MoS₂ and 2D nanosheet MoS₂ was investigated using organisms of four different taxonomic groups. Ultrasound-assisted liquid-phase exfoliation method was used for preparing 2D nanosheets from bulk MoS₂. Bulk and nanosheet MoS₂ were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses. The acute toxicity of the bulk and nanosheet MoS₂ catalysts was evaluated with four different bioassays using the test organisms Vibrio fischeri (a marine photobacterium), Pseudokirchnerialla subcapitata (a freshwater microalga), Daphnia magna (a freshwater crustacean) and the freshwater duckweed Spirodela polyrhiza. The toxic effect of the materials depended on their structural/size features and the type/sensitivity of the test organism. Generally speaking, bulk MoS₂ was more toxic than its nanosheet form. The freshwater crustacean Daphnia magna appeared to be the most suitable, easy-to-handle, and at the same time sensitive test organism for bulk and nanosheet MoS₂ among the tested organisms.

Keywords: Bioassay; Bulk and nanosheet MoS(2); Catalyst characterization; Effective concentration (EC).