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. 2022 Nov;288(2):87-94.
doi: 10.1111/jmi.12977. Epub 2020 Nov 22.

Perspective of fibre-optical microendoscopy with microlenses

Baokai Wang  1 , Qiming Zhang  2 , Xi Chen  2 , Haitao Luan  2 , Min Gu  1   2
Affiliations

Perspective of fibre-optical microendoscopy with microlenses

Baokai Wang et al. J Microsc. 2022 Nov.

Abstract
in English, Spanish

Fibre-optical microendoscopy is based on fibre-optical confocal scanning microscopy, where optical fibres are introduced for delivery of the source and collection of the signal. Fibre-optical microendoscopy has led to innovations in imaging of freely moving animals, long-term imaging, minimally invasive diagnostics, and microsurgery. The lens system in fibre-optical microendoscopy is significant because of the imaging resolution and miniaturisation possibility. State-of-the-art fibre-optical microendoscopy based on various types of lens systems is introduced and compared. The lens system contains an objective lens, a gradient index microlens, and other novel lens systems fabricated by electric arc discharge or two-photon lithography.

Optical fibres are introduced in confocal scanning microscopy, inspiring the development of fibre-optical microendoscopy. Fibre-optical microendoscopy takes advantages in imaging of freely moving animals, long-term imaging, minimally invasive diagnostics, and microsurgery. The lens system helps resolving small details of samples and reducing the size in a fibre-optical microendoscope. Various types of lens systems applied in the fibre-optical microendoscopes are introduced and compared, including an objective lens, a gradient index microlens, and other nanofabricated microlens systems.

Keywords: fibre-optics; microendoscopy; lenses.

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References

REFERENCES

    1. Helmchen, F., Fee, M. S., Tank, D. W., & Denk, W. (2001). A miniature head-mounted two-photon microscope: High-resolution brain imaging in freely moving animals. Neuron, 31(6), 903-912. https://doi.org/10.1016/S0896-6273(01)00421-4.
    1. Benschop, J., & Rosmalen, G. V. (1991). Confocal compact scanning optical microscope based on compact disc technology. Applied Optics, 30(10), 1179-1184. https://doi.org/10.1364/AO.30.001179.
    1. Kimura, S., & Wilson, T. (1991). Confocal scanning optical microscope using single-mode fiber for signal detection. Applied Optics, 30(16), 2143-2150. https://doi.org/10.1364/AO.30.002143.
    1. Gu, M., Sheppard, C. J. R., & Gan, Xi (1991). Image formation in a fiber-optical confocal scanning microscope. JOSA A, 8(11), 1755-1761. https://doi.org/10.1364/JOSAA.8.001755.
    1. Gu, M. (1996). Principles of three dimensional imaging in confocal microscopes. Singapore: World Scientific. https://doi.org/10.1142/3014.

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