Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay

Affiliations

¹ Microbiological Diagnostic Unit, Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia; Department of Microbiology, Monash Health, Melbourne, Australia. Electronic address: Maryza.Graham@monashhealth.org.
² Department of Microbiology, Royal Melbourne Hospital, Melbourne, Australia.
³ Microbiological Diagnostic Unit, Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
⁴ The Pacific Community, Public Health Division, Suva, Fiji.
⁵ Victorian Infectious Diseases Reference Laboratory, Melbourne Health at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
⁶ Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty, Institute for Infection and Immunity, Melbourne, Australia.
⁷ Microbiological Diagnostic Unit, Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia; Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty, Institute for Infection and Immunity, Melbourne, Australia.
⁸ Microbiological Diagnostic Unit, Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia; Department of Microbiology, Royal Melbourne Hospital, Melbourne, Australia; Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty, Institute for Infection and Immunity, Melbourne, Australia.

PMID: 33171384
PMCID: PMC7558241
DOI: 10.1016/j.diagmicrobio.2020.115238

Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay

Maryza Graham et al. Diagn Microbiol Infect Dis. 2021 Feb.

. 2021 Feb;99(2):115238.

doi: 10.1016/j.diagmicrobio.2020.115238. Epub 2020 Oct 15.

Authors

Affiliations

¹ Microbiological Diagnostic Unit, Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia; Department of Microbiology, Monash Health, Melbourne, Australia. Electronic address: Maryza.Graham@monashhealth.org.
² Department of Microbiology, Royal Melbourne Hospital, Melbourne, Australia.
³ Microbiological Diagnostic Unit, Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
⁴ The Pacific Community, Public Health Division, Suva, Fiji.
⁵ Victorian Infectious Diseases Reference Laboratory, Melbourne Health at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
⁶ Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty, Institute for Infection and Immunity, Melbourne, Australia.
⁷ Microbiological Diagnostic Unit, Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia; Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty, Institute for Infection and Immunity, Melbourne, Australia.
⁸ Microbiological Diagnostic Unit, Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia; Department of Microbiology, Royal Melbourne Hospital, Melbourne, Australia; Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty, Institute for Infection and Immunity, Melbourne, Australia.

PMID: 33171384
PMCID: PMC7558241
DOI: 10.1016/j.diagmicrobio.2020.115238

Abstract

The COVID-19 pandemic has placed unprecedented global demand on laboratory supplies required for testing. Sample pooling has been investigated by laboratories as a strategy to preserve testing capacity. We evaluate the performance of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay for testing samples in pools of 4 and 6. Clinical samples containing SARS-CoV-2, and confirmed negative clinical samples were used to create sample pools. Clinical samples had 'neat' Xpert® E gene cycle threshold values ranging between 20 and 28 and all were detected qualitatively when contained in pools of 4 or 6 samples. For these samples, pooling had a median change in cycle threshold value of 2.0 in pools of 4, and of 2.9 in pools of 6. With the use of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay, pooling of 4 or 6 samples may be an effective strategy to increase testing capacity.

Keywords: Cepheid; GeneXpert; Pooling; SARS-CoV-2.

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Figures

Fig 1 — **Fig. 1**
Standard curve of Xpert E gene Ct value and viral load.

See this image and copyright information in PMC

References

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Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay

Affiliations

Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay

Authors

Affiliations

Abstract

Figures

References

MeSH terms

LinkOut - more resources

Full Text Sources

Other Literature Sources

Medical

Miscellaneous