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. 2020 Oct 15:8:589993.
doi: 10.3389/fcell.2020.589993. eCollection 2020.

gRASping Depolarization: Contribution of RAS GTPases to Mitotic Polarity Clusters Resolution

Roberto Quadri  1 , Sarah Sertic  1 , Marco Muzi-Falconi  1
Affiliations

gRASping Depolarization: Contribution of RAS GTPases to Mitotic Polarity Clusters Resolution

Roberto Quadri et al. Front Cell Dev Biol. .
No abstract available

Keywords: GTPase; Ras; Saccharomyces cerevisiae; depolarization; mitosis; phosphorylation.

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Figures

Figure 1
Figure 1
Model—In the early stages of the cell cycle, bud emergence and growth are promoted through the accumulation of polarity factors (e.g., Cdc24 and Lte1) at the presumptive bud site and later on at the bud tip, thanks to physical interaction with polarisome components. At the time of G2/M phase, however, some of these proteins need to be redistributed to the whole daughter cortex to prevent the detrimental effects of hyperpolarization. This redistribution is regulated by Cdc-Clb/PAK–mediated phosphorylation of such polarity factors, which acts as a molecular switch to promote their binding to the evenly distributed GTP-Ras, thereby leading to isotropic growth. At the end of the cell cycle, the phosphatase Cdc14 removes the phosphate groups, replenishing the cellular pool of polarization-promoting proteins.
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References

    1. Adhikari H., Counter C. M. (2018). Interrogating the protein interactomes of RAS isoforms identifies PIP5K1A as a KRAS-specific vulnerability. Nat. Commun. 9:3646. 10.1038/s41467-018-05692-6 - DOI - PMC - PubMed
    1. Ballou E. R., Kozubowski L., Nichols C. B., Alspaugh J. A. (2013). Ras1 Acts through duplicated Cdc42 and Rac proteins to regulate morphogenesis and pathogenesis in the human fungal pathogen Cryptococcus neoformans. PLoS Genet. 9:e1003687. 10.1371/journal.pgen.1003687 - DOI - PMC - PubMed
    1. Bose I., Irazoqui J. E., Moskow J. J., Bardes E. S. G., Zyla T. R., Lew D. J. (2001). Assembly of scaffold-mediated complexes containing Cdc42p, the exchange factor Cdc24p, and the effector Cla4p required for cell cycle-regulated phosphorylation of Cdc24p. J. Biol. Chem. 276, 7176–7186. 10.1074/jbc.M010546200 - DOI - PubMed
    1. Butler M. T., Wallingford J. B. (2017). Planar cell polarity in development and disease. Nat. Rev. Mol. Cell Biol. 18, 375–388. 10.1038/nrm.2017.11 - DOI - PMC - PubMed
    1. Butty A. C., Perrinjaquet N., Petit A., Jaquenoud M., Segall J. E., Hofmann K., et al. . (2002). A positive feedback loop stabilizes the guanine-nucleotide exchange factor Cdc24 at sites of polarization. EMBO J. 21, 1565–1576. 10.1093/emboj/21.7.1565 - DOI - PMC - PubMed

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