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. 2021:2189:19-30.
doi: 10.1007/978-1-0716-0822-7_2.

Computational Design of Multiplex Oligonucleotide-Based Assays

Michaela Hendling  1 , Ivan Barišić  2
Affiliations

Computational Design of Multiplex Oligonucleotide-Based Assays

Michaela Hendling et al. Methods Mol Biol. 2021.

Abstract

The success of any oligonucleotide-based experiment strongly depends on the accurate design of the components. Oli2go is a user-friendly web tool that provides efficient multiplex oligonucleotide design including specificity and primer dimer checks. Its fully automated workflow involves important design steps that use specific parameters to produce high-quality oligonucleotides. This chapter describes how these steps are computationally implemented by oli2go.

Keywords: Multiplex oligonucleotide design; Primer dimers; Probe specificity.

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