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. 2021 Feb;43(2):423-433.
doi: 10.1007/s10529-020-03026-5. Epub 2020 Nov 13.

Establishment and optimization of a high-throughput mimic perfusion model in ambr® 15

Lu Jin  1 , Zhen-Shou Wang  2 , Yun Cao  2 , Rui-Qiang Sun  2 , Hang Zhou  3 , Rong-Yue Cao  4
Affiliations

Establishment and optimization of a high-throughput mimic perfusion model in ambr® 15

Lu Jin et al. Biotechnol Lett. 2021 Feb.

Abstract

Objectives: To establish an automated high-throughput mimic perfusion scale-down model (SDM) in ambr® 15 system.

Results: An optimized SDM for mimic perfusion was developed in ambr® 15 system. Cell retention in ambr® 15 was realized by sedimentation and supernatant removal with a retention rate > 95%. Although the SDM couldn't reach the viable cell density (VCD) at a bench scale bioreactor (BR), it maintained VCD at approximately 30 × 106 cells/mL with a cell bleeding rate estimated theoretically and predicted the cell specific perfusion rate (CSPR). A base-feeding strategy was developed to alleviate the pH drop during sedimentation which would adversely have an impact on cell growth, and showed an apparent cell viability improvement from 79.6% (control) to 90.1% on Day 18. The optimized SDM for mimic perfusion was employed for media screening in two cell lines.

Conclusions: A small-scale high-throughput perfusion model in ambr® 15 was developed, optimized to improve cell viability, and as a result, utilized for media screening in two cell lines.

Keywords: Ambr® 15; High-throughput; Perfusion; Sedimentation; Viability.

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