Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Feb;43(2):423-433.
doi: 10.1007/s10529-020-03026-5. Epub 2020 Nov 13.

Establishment and optimization of a high-throughput mimic perfusion model in ambr® 15

Lu Jin  1 , Zhen-Shou Wang  2 , Yun Cao  2 , Rui-Qiang Sun  2 , Hang Zhou  3 , Rong-Yue Cao  4
Affiliations

Establishment and optimization of a high-throughput mimic perfusion model in ambr® 15

Lu Jin et al. Biotechnol Lett. 2021 Feb.

Abstract

Objectives: To establish an automated high-throughput mimic perfusion scale-down model (SDM) in ambr® 15 system.

Results: An optimized SDM for mimic perfusion was developed in ambr® 15 system. Cell retention in ambr® 15 was realized by sedimentation and supernatant removal with a retention rate > 95%. Although the SDM couldn't reach the viable cell density (VCD) at a bench scale bioreactor (BR), it maintained VCD at approximately 30 × 106 cells/mL with a cell bleeding rate estimated theoretically and predicted the cell specific perfusion rate (CSPR). A base-feeding strategy was developed to alleviate the pH drop during sedimentation which would adversely have an impact on cell growth, and showed an apparent cell viability improvement from 79.6% (control) to 90.1% on Day 18. The optimized SDM for mimic perfusion was employed for media screening in two cell lines.

Conclusions: A small-scale high-throughput perfusion model in ambr® 15 was developed, optimized to improve cell viability, and as a result, utilized for media screening in two cell lines.

Keywords: Ambr® 15; High-throughput; Perfusion; Sedimentation; Viability.

PubMed Disclaimer

References

    1. Amanullah A et al (2010) Novel micro-bioreactor high throughput technology for cell culture process development: reproducibility and scalability assessment of fed-batch CHO cultures. Biotechnol Bioeng 106:57–67 - PubMed
    1. Bareither R, Pollard D (2011) A review of advanced small-scale parallel bioreactor technology for accelerated process development: current state and future need. Biotechnol Prog 27:2–14 - DOI
    1. Bielser J-M, Wolf M, Souquet J, Broly H, Morbidelli M (2018) Perfusion mammalian cell culture for recombinant protein manufacturing—a critical review. Biotechnol Adv 36:1328–1340 - DOI
    1. Butler M (2005) Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals. Appl Microbiol Biotechnol 68:283–291 - DOI
    1. Charaniya S, Le H, Rangwala H, Mills K, Johnson K, Karypis G, Hu W-S (2010) Mining manufacturing data for discovery of high productivity process characteristics. J Biotechnol 147:186–197 - DOI

LinkOut - more resources