In vitro studies on normal, stimulated and immunologically activated mouse macrophages. I. Oxidation of 1-14C glucose by macrophages in monolayer cultures

Abstract

Oxidation of 1-14C glucose of BCG/listeria activated, proteose-peptone stimulated and typhoid-parathypoid vaccine (T.A.B.) stimulated peritoneal and spleen macrophages from C3H, CBA and C57Bl mice was determined at various times after treatment. The study showed no major differences between the oxidation of stimulated and activated macrophages, but a variation was seen in C3H mice in the kinetics of glucose oxidation of BCG/listeria activated and proteose-peptone stimulated peritoneal macrophages. It was also observed, in these mice, that the response appeared and disappeared earlier in the spleen than in the peritoneal macrophages.