Inhibition of fibroblast proliferation in a culture of human endometrial stromal cells using a medium containing D-valine

Abstract

A nutrient medium in which D-valine was substituted for L-valine inhibited fibroblast proliferation in a culture of human endometrial stromal cells. Fibroblasts were not killed by D-valine and were able to grow again when D-valine was replaced by L-valine. The stromal cells proliferate in the D-valine medium only when seeded at high density. They were distinguished from fibroblasts by their morphology in light microscopy, their surface characteristics at scanning electron microscopy and their lower staining with fibronectin antibodies.