Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Clinical Trial
. 2020 Dec:175:106188.
doi: 10.1016/j.rmed.2020.106188. Epub 2020 Nov 7.

Efficacy of therapeutic plasma exchange in the treatment of penn class 3 and 4 cytokine release syndrome complicating COVID-19

W Larry Gluck  1 , Sean P Callahan  2 , Robert A Brevetta  2 , Antine E Stenbit  2 , Wesley M Smith  2 , Julie C Martin  2 , Anna V Blenda  3 , Sergio Arce  3 , W Jeffery Edenfield  2
Affiliations
Clinical Trial

Efficacy of therapeutic plasma exchange in the treatment of penn class 3 and 4 cytokine release syndrome complicating COVID-19

W Larry Gluck et al. Respir Med. 2020 Dec.

Abstract

Objectives: Cytokine release syndrome (CRS) is a potentially severe complication of COVID-19 most commonly resulting in respiratory failure. This ten-patient study was designed to determine the efficacy of therapeutic plasma exchange (TPE) in improving oxygenation and in reducing the cytokine load in a critically ill subset of patients.

Methods: Five single volume plasma exchanges over eight days within a 14-day study period. In mechanically ventilated patients, oxygenation was measured via the PaO2/FiO2 (P/F) ratio and the oxygenation index (OI) daily for 14 days. Supplemental oxygen requirements were tracked daily for non-ventilated patients.

Results: Non-ventilated patients were liberated from supplemental oxygen after TPE. The response was rapid with an 87% average reduction in oxygenation requirements following and average time to return to room air of 5.25 days. All mechanically ventilated patients demonstrated improvement in oxygenation with a 78% average improvement in the P/F ratio and a 43% improvement in OI. C-reactive protein (CRP) and serum levels of IL-6, IL-8, IL-10, TNFα, IFNγ and GM-CSF, were measured daily with immediate post TPE levels drawn on days 1, 2, 4, 6 and 8. All patients demonstrated significant reductions in CRP, IL-6, IL-10 and TNFα.

Conclusions: In the majority of patients with Penn class 3 and 4 CRS complicating COVID-19, TPE demonstrated a prompt improvement in oxygenation and reduction in cytokine load without compromising patient safety. As this pilot study was envisioned to be hypothesis generating, expanded trials using TPE alone and in conjunction with novel pharmacologic agents are warranted.

Registration: ClinicalTrials.gov NCT04374149.

Keywords: COVID-19; Cytokine release syndrome; Oxygenation; Respiratory failure; Therapeutic benefit; Therapeutic plasma exchange.

PubMed Disclaimer

Conflict of interest statement

The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: W. Jeffery Edenfield serves as a consultant for Chimerix, Inc., unrelated to the submitted work. The remaining authors have disclosed that they do not have any conflicts of interest.

Figures

Fig. 1
Fig. 1
Two-sample Median Comparisons for CRP and Cytokine Levels. The median of CRP and cytokine levels was compared in all ten patients following the second and fifth (final) plasma exchange and at day 14. a Non-cardiac (inflammatory) CRP reference range. b Cytokine levels represent average concentrations + standard deviation [Kim et al. J TranslMed 9,113 (2011) https://doi.org/10.1186/1479-5876-9-113].
See this image and copyright information in PMC

References

    1. Zhou P., Yang X., Wang X., et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020;579:270–273. - PMC - PubMed
    1. Novel Coronavirus Situation Report-2. World Health Organization; Geneva: January 2020. https://www.who.int/docs/default-source/coronavirus/situation-reports/20...
    1. Transmission of SARS-CoV-2: Implications for Infection Prevention Precautions. World Health Organization; Geneva: July 2020. https://www.who.int/publications/i/item/modes-of-transmission-of-virus-c...
    1. Padmanabhan A., Connelly-Smith L., Aqui N., et al. Guidelines on the use of therapeutic apheresis in clinical practice- evidence-based approach from the writing committee of the American Society for Apheresis: the eighth special issue. J. Chromatogr., A. 2019;34(3):167–354. - PubMed
    1. Busund R., Koukline V., Utrobin U., et al. Plasmapheresis in severe sepsis and septic shock: a prospective, randomised, controlled trial. Intensive Care Med. 2002;28:1434–1439. - PubMed

Publication types

MeSH terms

Associated data