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. 2020 Oct 22:11:602495.
doi: 10.3389/fgene.2020.602495. eCollection 2020.

Identification and Validation of a Novel Major Quantitative Trait Locus for Plant Height in Common Wheat (Triticum aestivum L.)

Zhiqiang Wang  1 , Haiyan Hu  2 , Xiaojun Jiang  1 , Yang Tao  3 , Yu Lin  1 , Fangkun Wu  1 , Shuai Hou  1 , Shihang Liu  1 , Caixia Li  1 , Guangdeng Chen  4 , Yaxi Liu  1   5
Affiliations

Identification and Validation of a Novel Major Quantitative Trait Locus for Plant Height in Common Wheat (Triticum aestivum L.)

Zhiqiang Wang et al. Front Genet. .

Abstract

Plant height (PH) plays a pivotal role in plant morphological architecture and is associated with yield potential in wheat. For the quantitative trait locus (QTL) analysis, a recombinant inbred line population was developed between varieties differing significantly in PH. Two major QTL were identified on chromosomes 4B (QPh.sicau-4B) and 6D (QPh.sicau-6D) in multiple environments, which were then validated in two different backgrounds by using closely linked markers. QPh.sicau-4B explained 10.1-21.3% of the phenotypic variance, and the location corresponded to the dwarfing gene Rht-B1. QPh.sicau-6D might be a novel QTL for PH, explaining 6.6-13.6% of the phenotypic variance and affecting spike length, thousand-kernel weight, and spikelet compactness. Three candidate genes associated with plant growth and development were identified in the physical interval of QPh.sicau-6D. Collectively, we identified a novel stable and major PH QTL, QPh.sicau-6D, which could aid in the development of closely linked markers for marker-assisted breeding and cloning genes underlying this QTL.

Keywords: candidate gene; plant height; quantitative trait locus; validation; wheat.

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Figures

FIGURE 1
FIGURE 1
Effects of the QPh.sicau-4B and QPh.sicau-6D on PH in HCN population (A: carrying both the additive alleles of the two major QTL; B: only carrying the additive allele of QPh.sicau-4B; C: only carrying the additive allele of QPh.sicau-6D; and D: not carrying the additive alleles of the two major QTL). **indicates significant differences at P < 0.01.
FIGURE 2
FIGURE 2
Genetic map of the major QTL QPh.sicau-4B with the marker KASP-4B.
FIGURE 3
FIGURE 3
Genetic map of the major QTL QPh.sicau-6D with the marker KASP-6D.
FIGURE 4
FIGURE 4
The genes expression of in various tissues in the QPh.sicau-4B interval and QPh.sicau-6D interval from the expVIP Wheat Expression Brower (A: physical map of chromosome 4B and chromosome 6D, B: root, C: leaf, D: spike, E: grain).
FIGURE 5
FIGURE 5
Sequence analysis of the candidate genes showing the SNPs and Indels between H461 and CN16. The nucleotide of H461 and CN16 are shown in red and black, respectively (A: Rht-1B, B: TraesCS6D02G227300, C: TraesCS6D02G233000).
See this image and copyright information in PMC

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