. 2020 Dec;6(12):1432-1438.

doi: 10.1038/s41477-020-00800-1. Epub 2020 Nov 16.

Structural insights into the photoactivation of Arabidopsis CRY2

Ling Ma^{1

2

3}, Zeyuan Guan¹, Qiang Wang¹, Xuhui Yan¹, Jing Wang¹, Zhizheng Wang⁴, Jianbo Cao⁵, Delin Zhang¹, Xin Gong⁶, Ping Yin⁷

Affiliations

¹ National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, China.
² Kobilka Institute of Innovative Drug Discovery, The Chinese University of Hong Kong, Shenzhen, Shenzhen, China.
³ School of Life Sciences, University of Science and Technology of China, Hefei, China.
⁴ Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, P. R. China.
⁵ Public Laboratory of Electron Microscopy, Huazhong Agricultural University, Wuhan, China.
⁶ Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, China. gongx@sustech.edu.cn.
⁷ National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, China. yinping@mail.hzau.edu.cn.

PMID: 33199893
DOI: 10.1038/s41477-020-00800-1

Structural insights into the photoactivation of Arabidopsis CRY2

Ling Ma et al. Nat Plants. 2020 Dec.

. 2020 Dec;6(12):1432-1438.

doi: 10.1038/s41477-020-00800-1. Epub 2020 Nov 16.

Authors

Ling Ma^{1

2

3}, Zeyuan Guan¹, Qiang Wang¹, Xuhui Yan¹, Jing Wang¹, Zhizheng Wang⁴, Jianbo Cao⁵, Delin Zhang¹, Xin Gong⁶, Ping Yin⁷

Affiliations

¹ National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, China.
² Kobilka Institute of Innovative Drug Discovery, The Chinese University of Hong Kong, Shenzhen, Shenzhen, China.
³ School of Life Sciences, University of Science and Technology of China, Hefei, China.
⁴ Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, P. R. China.
⁵ Public Laboratory of Electron Microscopy, Huazhong Agricultural University, Wuhan, China.
⁶ Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, China. gongx@sustech.edu.cn.
⁷ National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, China. yinping@mail.hzau.edu.cn.

PMID: 33199893
DOI: 10.1038/s41477-020-00800-1

Abstract

The blue-light receptor cryptochrome (CRY) in plants undergoes oligomerization to transduce blue-light signals after irradiation, but the corresponding molecular mechanism remains poorly understood. Here, we report the cryogenic electron microscopy structure of a blue-light-activated CRY2 tetramer at a resolution of 3.1 Å, which shows how the CRY2 tetramer assembles. Our study provides insights into blue-light-mediated activation of CRY2 and a theoretical basis for developing regulators of CRYs for optogenetic manipulation.

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References

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1. Wang, Q. & Lin, C. T. Mechanisms of cryptochrome-mediated photoresponses in plants. Annu. Rev. Plant Biol. 71, 23.1–23.27 (2020). - DOI
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1. Shao, K. et al. The oligomeric structures of plant cryptochromes. Nat. Struct. Mol. Biol. 27, 480–488 (2020). - DOI

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Structural insights into the photoactivation of Arabidopsis CRY2

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