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. 2021 Aug;93(8):4780-4785.
doi: 10.1002/jmv.26666. Epub 2021 Mar 14.

Identification of human parvovirus 4 genotypes 1 and 2 in Chinese source plasma pools

Junting Jia  1   2 , Yadi Zhong  2   3 , Huan Zhang  2 , Dian Yuan  2 , Limin Ma  2 , Deqing Wang  1 , Jingang Zhang  2 , Yuyuan Ma  2
Affiliations

Identification of human parvovirus 4 genotypes 1 and 2 in Chinese source plasma pools

Junting Jia et al. J Med Virol. 2021 Aug.

Abstract

Human parvovirus B19 (B19V) and human parvovirus 4 (PARV4) are known to infect humans and transmit through contaminated blood and blood products. Globally, three genotypes of B19V, as well as PARV4, have been identified, respectively. The existence of different B19V genotypes in Chinese plasma donors has been investigated, however, the data regarding PARV4 were not available. The main objective of this study is to identify the genotypes of PARV4 circulating in Chinese plasma donors. By using a duplex quantitative polymerase chain reaction assay adapted for all genotypes of B19V and PARV4, 78 source plasma pools for fractionation were screened and quantified. Results showed that positive rates of B19V and PARV4 DNA in plasma pool samples were 25.64% and 14.10%, respectively. PARV4 sequences in two positive samples were next genotyped, and these two sequences belonged to PARV4 genotypes 1 and 2, respectively. In conclusion, the data present demonstrate the existence of PARV4 genotypes 1 and 2 in Chinese plasma donors for the first time and also show the relatively lower prevalence and level of PARV4 DNA in Chinese plasma donors in comparison with that of B19V DNA.

Keywords: genotype; human parvovirus 4; human parvovirus B19; quantitative PCR; source plasma pools.

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Conflict of interest statement

The authors declare that there are no conflict of interests.

Figures

Figure 1
Figure 1
Distribution of the B19V and PARV4 DNA load in virus DNA‐positive source plasma pools. B19V, human parvovirus B19; PARV4, human parvovirus 4
Figure 2
Figure 2
Phylogenetic analysis of human parvovirus 4 (PARV4) nucleotide sequences. The phylogenetic tree was constructed based on the 161‐nt NS1 region of PARV4 and the neighbor‐joining algorithm using the Kimura two‐parameter model. Two PARV4 sequences from this study (labeled with black circles) and a set of PARV4 sequences downloaded from GenBank (labeled with their GenBank accession number and isolate or strain name) used as references for the different genotypes were analyzed. Bootstrap replication frequencies are indicated above each node. Branch lengths are drawn to scale
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