doi: 10.1083/jcb.105.6.2649.
Import of fructose bisphosphate aldolase into the glycosomes of Trypanosoma brucei
Affiliations
- PMID: 3320052
- PMCID: PMC2114687
- DOI: 10.1083/jcb.105.6.2649
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Import of fructose bisphosphate aldolase into the glycosomes of Trypanosoma brucei
J Cell Biol.
1987 Dec.
Abstract
The glycolytic enzymes of Trypanosomatids are compartmentalized within peroxisome-like microbodies called glycosomes. Fructose bisphosphate aldolase is synthesized on free polysomes and imported into glycosomes within 5 min. Peptide mapping reveals no primary structural differences between the in vivo-synthesized protein and that made in vitro from a synthetic template. However, native aldolase from glycosomes is partially protease resistant, whereas the in vitro translation product is not. Pulse-chase results indicate that aldolase in bloodstream trypanosomes has a much longer half-life than in the procyclic tsetse fly form.
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