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. 2020 Nov 16;25(22):5332.
doi: 10.3390/molecules25225332.

Recovery of a Bacteriocin-Like Inhibitory Substance from Lactobacillus bulgaricus FTDC 1211 Using Polyethylene-Glycol Impregnated Amberlite XAD-4 Resins System

Nur Fazrin Husna Abdul Aziz  1 , Sahar Abbasiliasi  2 , Zhang Jin Ng  1 , Mazni Abu Zarin  1   3 , Siti Nurbaya Oslan  4 , Joo Shun Tan  1   5 , Arbakariya Bin Ariff  5   6
Affiliations

Recovery of a Bacteriocin-Like Inhibitory Substance from Lactobacillus bulgaricus FTDC 1211 Using Polyethylene-Glycol Impregnated Amberlite XAD-4 Resins System

Nur Fazrin Husna Abdul Aziz et al. Molecules. .

Abstract

Lactobacillus bulgaricus is a LAB strain which is capable of producing bacteriocin substances to inhibit Staphylococcus aureus. The aim of this study was to purify a bacteriocin-like inhibitory substance (BLIS) produced by L. bulgaricus FTDC 1211 using an aqueous impregnated resins system consisting of polyethylene-glycol (PEG) impregnated on Amberlite XAD4. Important parameters influencing on purification of BLIS, such as the molecular weight and concentration of PEG, the concentration and pH of sodium citrate and the concentration of sodium chloride, were optimized using a response surface methodology. Under optimum conditions of 11% (w/w) of PEG 4000 impregnated Amberlite XAD4 resins and 2% (w/w) of sodium citrate at pH 6, the maximum purification factor (3.26) and recovery yield (82.69% ± 0.06) were obtained. These results demonstrate that AIRS could be used as an alternate purification system in the primary recovery step.

Keywords: bacteriocin-like inhibitory substance; lactic acid bacteria; polymer; purification; resin.

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Conflict of interest statement

The authors have declared no conflict of interest.

Figures

Figure 1
Figure 1
Antimicrobial activity of (a1) 90% (v/v) of CCFS from L. bulgaricus FTDC1211, (a2,a3) 100% (v/v) of CCFS from L. bulgaricus FTDC1211 in duplicate and (b) control with 15 mg/mL of streptomycin against S. aureus.
Figure 2
Figure 2
The purification factor and recovery yield achieved for different concentration of sodium citrate (A), pH of sodium citrate (B) and NaCl (C). The results reported were expressed as a mean of triplicate reading with an estimated error of ±5%.
Figure 2
Figure 2
The purification factor and recovery yield achieved for different concentration of sodium citrate (A), pH of sodium citrate (B) and NaCl (C). The results reported were expressed as a mean of triplicate reading with an estimated error of ±5%.
Figure 3
Figure 3
3D surface plot for purification factor obtained for the combination effect of (A) concentration of PEG 4000 and pH of sodium citrate, (B) concentration of PEG 4000 and sodium citrate concentration and (C) pH and concentration of sodium citrate.
Figure 4
Figure 4
SDS-PAGE profile. Lane 1: standard marker with molecular weight of 10 to 180 kDa. Lane 2: partially purified BLIS from L. bulgaricus FTDC1211.
See this image and copyright information in PMC

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