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. 2020 Oct;52(3):271-276.
doi: 10.5152/eurasianjmed.2020.20008.

Leucocytes and Th-associated Cytokine Profile of HIV-Leishmaniasis Co-Infected Persons Attending Abuja Teaching Hospital, Nigeria

Idris Nasir Abdullahi  1 , Anthony Uchenna Emeribe  2 , Hafeez Aderinsayo Adekola  3 , Habiba Yahaya Muhammad  4 , Abdurrahman El-Fulaty Ahmad  1 , Abubakar Umar Anka  1 , Yusuf Mohammed  5 , Shamsuddeen Haruna  1 , Bamidele Soji Oderinde  6 , Halima Ali Shuwa  7 , Adamu Babayo  5
Affiliations

Leucocytes and Th-associated Cytokine Profile of HIV-Leishmaniasis Co-Infected Persons Attending Abuja Teaching Hospital, Nigeria

Idris Nasir Abdullahi et al. Eurasian J Med. 2020 Oct.

Abstract

Objective: T-helper cells (Th)-1& -2 cytokines homeostasis control or predict clinical outcome of infected persons, especially those with HIV /AIDS. This case-control study evaluated the leucocytes differentials, TNF-alpha, interleukin (IL)-2 and -10 levels among HIV infected persons with serological evidence of leishmaniasis attending University of Abuja Teaching Hospital, Nigeria.

Materials and methods: Blood samples from 28 HIV infected persons who had Leishmania donovani rK39 and Immunoglobulin-G (IgG) positive (group 1), 30 age- & -sex matched HIV infected persons without Leishmania antibodies (group 2) and 30 apparently healthy persons without HIV and Leishmania antibodies (group 3). Full blood counts, TNF alpha, IL-2 and -10 levels were analyzed using automated hematology analyzer and ELISA, respectively. Structured questionnaires were used to collate biodata and clinical presentations of participants.

Results: Ten (35.7%) participants in group 1 were on ART, 15 (50%) in group 2 were on ART, while group 3 were ART naïve. There were significantly higher values in basophil (4.4±2.5%) and eosinophil counts (12.9±3.8%) in HIV/leishmania coinfected persons (p<0.005). However, other white cells subpopulation was significantly lower in HIV/leishmania co-infected participants (p<0.05). There was significantly reduced CD4+ T cell counts ([119±26 versus 348±63 versus 605±116 cells/mm3]), TNF-alpha ([36.82±8.21 versus 64.67±12.54 versus 254.98±65.59 pg/mL]) and IL-2 levels ([142.14±20.91 versus 507.6±84.42 versus 486.62±167.87 pg/mL]) among HIV/Leishmania co-infected participants compared to group 2 and group 3 participants, respectively. However, higher IL-10 level (80.35±14.57 pg/mL) was found in HIV/Leishmania co-infected participants as opposed to the HIV monoinfected (62.2±10.43 pg/mL) and apparently healthy persons (23.97±4.88 pg/mL) (p<0.001).

Conclusion: Eosinophil, basophil counts and serum IL-10 level were high in HIV/Leishmania coinfected persons, demonstrating parasite-induced hypersensitivity and immunosuppression.

Keywords: Cellular immunity; HIV coinfection; cytokines; leishmaniasis; proinflammation.

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Conflict of interest statement

Conflict of Interest: Authors have no conflicts of interest to declare.

References

    1. Adriaensen W, Dorlo TPC, Vanham G, Kestens L, Kaye PM, van Griensven J. Immunomodulatory Therapy of Visceral Leishmaniasis in Human Immunodeficiency Virus-Coinfected Patients. Front Immunol. 2018;8:1943. doi: 10.3389/fimmu.2017.01943. - DOI - PMC - PubMed
    1. Monge-Maillo B, Norman FF, Cruz I, Alvar J, Lo’pez-Ve’lez R. Visceral Leishmaniasis and HIV Coinfection in the Mediterranean Region. PLoS Negl Trop Dis. 2014;8:e3021. doi: 10.1371/journal.pntd.0003021. - DOI - PMC - PubMed
    1. Lindoso JA, Cota GF, da Cruz AM, Goto H, Maia-Elkhoury ANS. Visceral Leishmaniasis and HIV Coinfection in Latin America. PLoS Negl Trop Dis. 2014;8:e3136. doi: 10.1371/journal.pntd.0003136. - DOI - PMC - PubMed
    1. Ezra N, Ochoa MT, Craft N. Human immunodeficiency virus and leishmaniasis. J Global Infect Dis. 2010;2:248–57. doi: 10.4103/0974-777X.68528. - DOI - PMC - PubMed
    1. de Vries HJC, Reedijk SH, Schallig HDFH. Cutaneous Leishmaniasis: Recent Developments in Diagnosis and Management. Am J Clin Dermatol. 2015;16:99–109. doi: 10.1007/s40257-015-0114-z. - DOI - PMC - PubMed

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