Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Dec 10;15(23):9945-56.
doi: 10.1093/nar/15.23.9945.

Mutational analysis of upstream sequences required for transcriptional activation of the Klebsiella pneumoniae nifH promoter

Affiliations
Free PMC article

Mutational analysis of upstream sequences required for transcriptional activation of the Klebsiella pneumoniae nifH promoter

M Buck et al. Nucleic Acids Res. .
Free PMC article

Abstract

Upstream sequences of the Klebsiella pneumoniae nifH promoter were mutagenised and activation of the mutated promoters by the nif-specific transcriptional activator protein NifA examined in vivo. Of the sixteen mutations analysed, only those within the nifH upstream activator sequence (UAS), characterised by a TGT-N10-ACA motif, influenced nifH promoter activity. Mutations altering the two-fold rotational symmetry of the UAS or the spacing between the TGT and ACA motifs reduced promoter activity, consistent with the UAS functioning as a NifA binding site. The bases flanking the TGT-ACA motif of the UAS also appear to influence activation by NifA. Substituting the nifH UAS with a binding site for the transcriptional activator NtrC resulted in improved NtrC-dependent activation of the nifH promoter demonstrating that the activator specificity of the nifH promoter is dependent upon the presence of the appropriate upstream sequences to which the activator binds.

PubMed Disclaimer

References

    1. J Bacteriol. 1980 Aug;143(2):971-80 - PubMed
    1. Mol Microbiol. 1987 Sep;1(2):243-9 - PubMed
    1. J Bacteriol. 1983 Jan;153(1):45-56 - PubMed
    1. Annu Rev Genet. 1982;16:135-68 - PubMed
    1. J Bacteriol. 1984 Jan;157(1):143-7 - PubMed