doi: 10.1073/pnas.84.24.8839.
Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae
Affiliations
- PMID: 3321057
- PMCID: PMC299646
- DOI: 10.1073/pnas.84.24.8839
Item in Clipboard
Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae
Proc Natl Acad Sci U S A.
1987 Dec.
Abstract
A yeast nuclear extract supported transcription from the CYC1 and PYK1 promoters. Transcription was initiated in vitro at or near sites used in vivo. Deletion of "TATA" sequences abolished the reaction. alpha-Amanitin (10 micrograms/ml) and chloride (100 mM) were highly inhibitory.
Similar articles
-
Initiation by yeast RNA polymerase II at the adenoviral major late promoter in vitro.Science. 1989 Nov 3;246(4930):661-4. doi: 10.1126/science.2510298. Science. 1989. PMID: 2510298
-
Initiation on chromatin templates in a yeast RNA polymerase II transcription system.Genes Dev. 1992 Dec;6(12A):2282-7. doi: 10.1101/gad.6.12a.2282. Genes Dev. 1992. PMID: 1459452
-
Function of a yeast TATA element-binding protein in a mammalian transcription system.Nature. 1988 Jul 7;334(6177):37-42. doi: 10.1038/334037a0. Nature. 1988. PMID: 3290687
-
How does III x II make U6?Science. 1991 Dec 6;254(5037):1462-3. doi: 10.1126/science.1962205. Science. 1991. PMID: 1962205 Review. No abstract available.
-
The transcriptional apparatus required for mRNA encoding genes in the yeast Saccharomyces cerevisiae emerges from a jigsaw puzzle of transcription factors.FEMS Microbiol Rev. 1996 Dec;19(2):117-36. doi: 10.1111/j.1574-6976.1996.tb00256.x. FEMS Microbiol Rev. 1996. PMID: 8988567 Review.
Cited by
-
Mutations in RNA polymerase II enhance or suppress mutations in GAL4.Proc Natl Acad Sci U S A. 1989 Apr;86(8):2794-8. doi: 10.1073/pnas.86.8.2794. Proc Natl Acad Sci U S A. 1989. PMID: 2495535 Free PMC article.
-
Molecular genetics of the RNA polymerase II general transcriptional machinery.Microbiol Mol Biol Rev. 1998 Jun;62(2):465-503. doi: 10.1128/MMBR.62.2.465-503.1998. Microbiol Mol Biol Rev. 1998. PMID: 9618449 Free PMC article. Review.
-
Dynamics of interaction of RNA polymerase II with nucleosomes. I. Effect of salts.Protein Sci. 1993 Dec;2(12):2233-45. doi: 10.1002/pro.5560021223. Protein Sci. 1993. PMID: 8298467 Free PMC article.
-
Purification and lipid-layer crystallization of yeast RNA polymerase II.Proc Natl Acad Sci U S A. 1990 Mar;87(6):2122-6. doi: 10.1073/pnas.87.6.2122. Proc Natl Acad Sci U S A. 1990. PMID: 2179949 Free PMC article.
-
RNA polymerase II subunit composition, stoichiometry, and phosphorylation.Mol Cell Biol. 1990 May;10(5):1915-20. doi: 10.1128/mcb.10.5.1915-1920.1990. Mol Cell Biol. 1990. PMID: 2183013 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous
