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. 1987 Dec;84(24):8839-43.
doi: 10.1073/pnas.84.24.8839.

Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae

N F Lue  1 , R D Kornberg
Affiliations

Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae

N F Lue et al. Proc Natl Acad Sci U S A. 1987 Dec.

Abstract

A yeast nuclear extract supported transcription from the CYC1 and PYK1 promoters. Transcription was initiated in vitro at or near sites used in vivo. Deletion of "TATA" sequences abolished the reaction. alpha-Amanitin (10 micrograms/ml) and chloride (100 mM) were highly inhibitory.

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References

    1. J Bacteriol. 1983 Jan;153(1):163-8 - PubMed
    1. J Biol Chem. 1983 Feb 25;258(4):2193-201 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 May;80(9):2432-6 - PubMed
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