Simultaneous gelatin embedding of multiple areas of the arterial tree

Abstract

An embedding method for simultaneously cutting frozen sections of multiple segments of the arterial tree is presented. The segments are stacked in a standardized form and embedded in gelatin. Preparation of the blocks, cutting on a freezing microtome and storage of remnants are discussed. Alignment of the segments was easily achieved and the blocks were sectioned without difficulty. This technique saves space and effort and facilitates screening and rapid reading of slides.