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. 2021 Mar;48(3):403-410.
doi: 10.1111/cup.13924. Epub 2020 Dec 14.

Utility of YAP1 and NUT immunohistochemistry in the diagnosis of porocarcinoma

Eleanor Russell-Goldman  1 , Jason L Hornick  1 , John Hanna  1
Affiliations

Utility of YAP1 and NUT immunohistochemistry in the diagnosis of porocarcinoma

Eleanor Russell-Goldman et al. J Cutan Pathol. 2021 Mar.

Abstract

Background: Porocarcinoma is the malignant counterpart of poroma, a benign tumor derived from the eccrine or apocrine units. In contrast to poroma, porocarcinoma is rare and its diagnosis may be challenging. Recent work has identified YAP1-associated gene fusions in most poromas, and a subset of porocarcinomas. These included YAP1-MAML2 and YAP1-NUTM1, the latter being enriched in porocarcinomas over poromas.

Methods: We studied YAP1 C-terminus and NUT immunohistochemistry in a cohort of 12 porocarcinomas, 10 poromas, 10 squamous cell carcinomas, and 6 hidradenocarcinomas.

Results: Seven of 12 (58%) porocarcinomas showed loss of YAP1 C-terminus expression, consistent with a YAP1 fusion. Of these seven, five showed NUT positivity, implying the presence of the YAP1-NUTM1 fusion. One of 12 (8%) cases showed NUT positivity, but retention of YAP1 C-terminus expression, consistent with a non-YAP1 NUT-associated fusion. Eight of 10 (80%) poromas showed loss of YAP1 C-terminus expression and negative NUT staining, consistent with non-NUT YAP1 fusions. All squamous cell carcinomas and hidradenocarcinomas retained YAP1 C-terminus expression and were negative for NUT.

Conclusion: YAP1 C-terminus and NUT immunohistochemistry may be helpful in the diagnosis of porocarcinoma, with the combination of YAP1 C-terminus loss and NUT positivity being particularly informative.

Keywords: NUT; YAP1; porocarcinoma; poroma; squamous cell carcinoma.

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References

REFERENCES

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