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. 1987 Nov;6(11):3317-24.
doi: 10.1002/j.1460-2075.1987.tb02652.x.

Purification of KBF1, a common factor binding to both H-2 and beta 2-microglobulin enhancers

O Yano  1 , J Kanellopoulos, M Kieran, O Le Bail, A Israël, P Kourilsky
Affiliations

Purification of KBF1, a common factor binding to both H-2 and beta 2-microglobulin enhancers

O Yano et al. EMBO J. 1987 Nov.

Abstract

An enhancer binding factor, designated KBF1, has been purified from the nuclear extract of mouse BW5147 thymoma cells by five column chromatography steps including a sequence-specific DNA affinity column. Gel retardation and footprint analysis have shown that purified KBF1 has a binding activity specific for both H-2 and beta 2-microglobulin enhancer sequences. After SDS-polyacrylamide gel electrophoresis of the most purified preparation a 48-kd protein showed, after elution and renaturation, a binding activity to both enhancer sequences. These findings suggest that the expression of both H-2 and beta 2-microglobulin genes utilizes a common regulatory mechanism.

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References

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