Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Nov 19;9(11):1696.
doi: 10.3390/foods9111696.

Novel Biodegradable Starch Film for Food Packaging with Antimicrobial Chicory Root Extract and Phytic Acid as a Cross-Linking Agent

Andrzej Jaśkiewicz  1 , Grażyna Budryn  1 , Agnieszka Nowak  2 , Magdalena Efenberger-Szmechtyk  2
Affiliations

Novel Biodegradable Starch Film for Food Packaging with Antimicrobial Chicory Root Extract and Phytic Acid as a Cross-Linking Agent

Andrzej Jaśkiewicz et al. Foods. .

Abstract

The aim of the study was to obtain and evaluate the properties of biodegradable starch film with the addition of phytic acid (0.05%) as a cross-linking agent and chicory root extract (1-5%) as an antimicrobial agent. To prepare biodegradable film, extracts from chicory root obtained with water or methanol were used. The content of bioactive compounds (sesquiterpene lactones and total polyphenols) was evaluated in chicory extracts. The antibacterial activity of the extracts was tested against Gram-negative bacteria (Pseudomonas fluorescens, Escherichia coli) and Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus) using the microculture method. The extracts acted as bacteriostatic agents, decreasing the growth rate (µmax), and extending the lag phase (tlag). The most sensitive bacterium in terms of film bacteriostatic activity was P. fluorescens; all extracts, irrespective of the solvent used, decreased its µmax value. S. aureus was the least sensitive. The obtained films were tested for their properties as food packaging (color, thickness, permeability, mechanical strength). Phytic acid improved the tensile strength and barrier properties of the films. The antimicrobial activity of the films was studied by the disk diffusion method against Gram-negative (P. fluorescens, E. coli) and Gram-positive (B. subtilis, S. aureus) bacteria, as well as fungi (Candida albicans, Aspergillus niger). The growth-inhibiting activity of each obtained film was observed for all tested microorganisms, and the most beneficial effect was observed for films with the 5% level of added extracts obtained with water. The growth-inhibiting activity for fungi, in particular for the yeast C. albicans, was low.

Keywords: antimicrobial activity; biodegradable film; chicory root; phytic acid; sesquiterpene lactones; starch.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Representative images showing structure of foil using a scanning electron microscope (SEM): (a) control, 50 g of starch and 40 g of glycerol dissolved in 1 L of water; (b) 50 g of starch, 40 g of glycerol, and 0.5 g of phytic acid dissolved in 1 L of water; (c) 50 g of starch, 40 g of glycerol, 0.5 g of phytic acid, and 10 g of chicory root water–enzyme extract dissolved in 1 L of water; (d) 50 g of starch, 40 g of glycerol, 0.5 g of phytic acid, and 20 g of chicory root water–enzyme extract dissolved in 1 L of water; (e) 50 g of starch, 40 g of glycerol, 0.5 g of phytic acid, and 50 g of chicory root water—enzyme extract dissolved in 1 L of water.
Figure 1
Figure 1
Representative images showing structure of foil using a scanning electron microscope (SEM): (a) control, 50 g of starch and 40 g of glycerol dissolved in 1 L of water; (b) 50 g of starch, 40 g of glycerol, and 0.5 g of phytic acid dissolved in 1 L of water; (c) 50 g of starch, 40 g of glycerol, 0.5 g of phytic acid, and 10 g of chicory root water–enzyme extract dissolved in 1 L of water; (d) 50 g of starch, 40 g of glycerol, 0.5 g of phytic acid, and 20 g of chicory root water–enzyme extract dissolved in 1 L of water; (e) 50 g of starch, 40 g of glycerol, 0.5 g of phytic acid, and 50 g of chicory root water—enzyme extract dissolved in 1 L of water.
See this image and copyright information in PMC

References

    1. Zhu F. Starch based Pickering emulsions: Fabrication, properties, and applications. Trends Food Sci. Technol. 2019;85:129–137. doi: 10.1016/j.tifs.2019.01.012. - DOI
    1. Park E.Y., Lim S.T. Characterization of waxy starches phosphorylated using phytic acid. Carbohydr. Polym. 2019;225:115225. doi: 10.1016/j.carbpol.2019.115225. - DOI - PubMed
    1. Wang D., Wang Y., Li T., Zhang S., Ma P., Shi D., Chen M., Dong W. A bio-based flame-retardant starch based on phytic acid. ACS Sustain. Chem. Eng. 2020;8:10265–10274. doi: 10.1021/acssuschemeng.0c03277. - DOI
    1. Oatway L., Vasanthan T., Helm J.H. Phytic acid. Food Rev. Int. 2001;17:419–431. doi: 10.1081/FRI-100108531. - DOI
    1. Kalali E.N., Zhang L., Shabestari M.E., Croyal J., Wang D.Y. Flame-Retardant wood polymer composites (WPCs) as potential fire safe bio-based materials for building products: Preparation, flammability and mechanical properties. Fire Saf. J. 2019;107:210–216. doi: 10.1016/j.firesaf.2017.11.001. - DOI

LinkOut - more resources