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. 2020 Nov 19;9(11):1148.
doi: 10.3390/antiox9111148.

Antioxidant Activity and Acteoside Analysis of Abeliophyllum distichum

Hak-Dong Lee  1 , Ji Hyun Kim  2 , Qi Qi Pang  3 , Pil-Mun Jung  4 , Eun Ju Cho  3 , Sanghyun Lee  1
Affiliations

Antioxidant Activity and Acteoside Analysis of Abeliophyllum distichum

Hak-Dong Lee et al. Antioxidants (Basel). .

Abstract

This study determined acteoside and its content in Abeliophyllum distichum via HPLC/UV and LC/ESI-MS to obtain insights into the potential use of this plant as an antioxidant agent. Moreover, 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (•OH), and O2- radical scavenging activity assays were performed to assess in vitro antioxidative activity. The DPPH, •OH, and O2- radical scavenging activities of A. distichum leaf EtOH extracts at a 250 μg/mL concentration were 88.32%, 94.48%, and 14.36%, respectively, whereas those of stem extracts at the same concentration were 88.15%, 88.99%, and 15.36%, respectively. The contents of acteoside in A. distichum leaves and stems were 162.11 and 29.68 mg/g, respectively. Acteoside was identified as the main antioxidant compound in A. distichum leaves, which resulted in DPPH, •OH, and O2- radical scavenging activities of 82.84%, 89.46%, and 30.31%, respectively, at a 25 μg/mL concentration. These results indicate that A. distichum leaves and stems containing the antioxidant acteoside can be used as natural ingredients for functional and nutritional supplements.

Keywords: Abeliophyllum distichum; DPPH; HPLC/UV; O2− radical; acteoside; hydroxyl radical.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Chemical structure of acteoside.
Figure 2
Figure 2
LC/ESI-MS data of A. distichum (LC chromatogram (a) and ESI-MS data (b)).
Figure 3
Figure 3
HPLC chromatograms of acteoside (a) and the EtOH extracts from A. distichum leaves (b) and stems (c) (330 nm).
See this image and copyright information in PMC

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