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. 2021 Jan;65(1):10-16.
doi: 10.1111/1348-0421.12861. Epub 2020 Dec 14.

Reduction of severe acute respiratory syndrome coronavirus-2 infectivity by admissible concentration of ozone gas and water

Takayuki Murata  1 , Satoshi Komoto  1 , Satoko Iwahori  1 , Jun Sasaki  1 , Hironori Nishitsuji  1 , Terumitsu Hasebe  2 , Kiyotaka Hoshinaga  3 , Yukio Yuzawa  4
Affiliations

Reduction of severe acute respiratory syndrome coronavirus-2 infectivity by admissible concentration of ozone gas and water

Takayuki Murata et al. Microbiol Immunol. 2021 Jan.

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is causing the global coronavirus disease 2019 (COVID-19) pandemic. Because complete elimination of SARS-CoV-2 appears difficult, decreasing the risk of transmission is important. Treatment with 0.1 and 0.05 ppm ozone gas for 10 and 20 hr, respectively, decreased SARS-CoV-2 infectivity by about 95%. The magnitude of the effect was dependent on humidity. Treatment with 1 and 2 mg/L ozone water for 10 s reduced SARS-CoV-2 infectivity by about 2 and 3 logs, respectively. Our results suggest that low-dose ozone, in the form of gas and water, is effective against SARS-CoV-2.

Keywords: SARS-CoV-2; TCID50; humidity; ozone gas; ozone water.

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Figures

Figure 1
Figure 1
Equipment for ozone gas administration. (a) Two containers were prepared: one for ozone gas treatment and another for control treatment. (b) Schematic diagram of the ozone gas equipment. The control system is switched on or off according to the data obtained by the gas‐measuring device. (c) Water reservoir for humidity [Color figure can be viewed at wileyonlinelibrary.com]
Figure 2
Figure 2
Relationship of the CT value of ozone gas with the tissue culture infectious dose 50 (TCID50) value. The average log TCID50/mL values of two or three independent experiments are shown
Figure 3
Figure 3
Relationship of the ozone concentration in water with the tissue culture infectious dose 50 (TCID50) value. The average log TCID50/mL value of three independent experiments is shown
See this image and copyright information in PMC

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