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. 2020 Nov 20;12(24):25819-25827.
doi: 10.18632/aging.104203. Epub 2020 Nov 20.

Association and functional study between ADIPOQ and metabolic syndrome in elderly Chinese Han population

Qiao Wang  1 , Decheng Ren  2 , Yan Bi  2 , Ruixue Yuan  2 , Dong Li  3 , Jianying Wang  1 , Ruirui Wang  1 , Lei Zhang  1 , Guang He  2 , Baocheng Liu  1
Affiliations

Association and functional study between ADIPOQ and metabolic syndrome in elderly Chinese Han population

Qiao Wang et al. Aging (Albany NY). .

Abstract

Objective: Metabolic syndrome (MetS) is a cluster of health problems that places individuals at higher risk of developing cardiovascular disease, diabetes and stroke. The prevalence of MetS is increasing worldwide. It is also well accepted that genetic and environmental factors play significant roles in the occurrence/development of MetS, but studies exploring genetic factors are still lacking. Here, we aimed to investigate the association of ADIPOQ gene variants with MetS in an elderly Chinese Han population.

Results: We found that the allelic frequencies of rs6773957 and rs3774261 were significantly different between MetS and the control (p = 0.031; p = 0.049). Furthermore, a reduction in luciferase activity was observed when HEK293T cells were transfected with rs6773957 mutant fragments compared with wild type.

Conclusion: Our results suggest that rs6773957 and rs3774261 of ADIPOQ were associated with MetS in the elderly Chinese Han population. The functional assays performed indicate that the rs6773957 variant might be pathogenic and may provide evidence for mechanistic studies of MetS in the future.

Methods: Four single nucleotide polymorphisms (SNPs) were selected and genotyped (rs6773957, rs182052, rs3774261 and rs17366568) in 1337 subjects, including 569 healthy controls and 768 MetS cases. The clinical characteristics of all the subjects were obtained and analyzed. Additionally, a functional study of rs6773957 in regulating the expression of ADIPOQ was performed in this study.

Keywords: 3' UTR; ADIPOQ; association; metabolic syndrome.

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Conflict of interest statement

CONFLICTS OF INTEREST: The author declared that there were no conflicts of interest.

Figures

Figure 1
Figure 1
LD structure of the four polymorphisms in the ADIPOQ gene with Haploview analysis. Red squares indicate significant statistical LD between the pair of polymorphisms (D’ > 80).
Figure 2
Figure 2
The luciferase activity of the two recombinant DNA plasmids ADIPOQ-A (wild type) and ADIPOQ-G (mutant type) transfected in HEK293 cells was graphed after normalization against the plasmid pmirGLO expressing the Renilla luciferase gene. Data for rs6773957 pmirGLO, pmirGLO-ADIPOQ-A and pmirGLO-ADIPOQ-G are presented as the mean and SD of n=9 samples. ***, p 0.001, one-way ANOVA test.
See this image and copyright information in PMC

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