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. 2020 Nov 20;10(11):2160.
doi: 10.3390/ani10112160.

Effects of Aging on Expression of Mic60 and OPA1 and Mitochondrial Morphology in Myocardium of Tibetan Sheep

Guan Wang  1   2 , Yuzhu Luo  1 , Jiang Hu  1 , Jiqing Wang  1 , Xiu Liu  1 , Shaobin Li  1
Affiliations

Effects of Aging on Expression of Mic60 and OPA1 and Mitochondrial Morphology in Myocardium of Tibetan Sheep

Guan Wang et al. Animals (Basel). .

Abstract

In order to investigate the effects of aging on the expression of Mic60 and OPA1 and mitochondrial morphology in plateau animals, the expression of Mic60 and OPA1 genes and proteins, and the morphology of mitochondria in the myocardium of adult and aged Tibetan sheep were investigated. The expression of Mic60 and OPA1 genes and OPA1 protein were higher (p < 0.05) in the myocardium of adult Tibetan sheep than in those of the aged ones. The number of mitochondrial cristae in the myocardium of adult was higher than that in aged (p < 0.05). The density of mitochondria in the myocardium of adult was higher than that in aged (p < 0.01). Compared with the adult Tibetan sheep, the mitochondrial crista of aged were relatively sparse, the crista membrane was wide, and the mitochondria were not closely linked, showing fragmentation. These results suggest that the myocardial mitochondria of the adult have better energy supply ability, indicating that aging can lead to the weakening of oxygen supply in the myocardial mitochondria of Tibetan sheep.

Keywords: Mic60; OPA1; Tibetan sheep; aging; myocardial mitochondria.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Melting curves of Mic60, OPA1 and β-actin genes and their relative expression in adult and aged Tibetan sheep cardiomyocytes. Note: (a,b): Melting curve of Mic60, OPA1 and β-actin genes. (c,d): Relative expression of Mic60 and OPA1 genes. All data showed as means ± SD (n = 3). ** p < 0.01.
Figure 2
Figure 2
Expression of Mic60 and OPA1 proteins in cardiomyocytes. Note: (a–c): Expression of Mic60 protein in adult and aged Tibetan sheep myocardium and blank control. (d–f): Expression of OPA1 protein in adult and aged Tibetan sheep myocardium and blank control. (g): Protein concentration of Mic60 and OPA1. Data show means ± SD (n = 3). * p < 0.05.
Figure 3
Figure 3
Ultrastructure observation of myocardial mitochondria in adult and aged Tibetan sheep. Note: (a,b): Adult and aged Tibetan sheep myocardial mitochondria, bar = 0.5 μm. (c,d): Mitochondria isolated from adult and aged Tibetan Sheep myocardium, bar = 0.2 μm.
Figure 4
Figure 4
The number of mitochondrial crista (a) and density of mitochondria (b) in the myocardium of adult and aged Tibetan Sheep. Data show means ± SD. * p < 0.05, ** p < 0.01.
See this image and copyright information in PMC

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