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. 2020 Jul 30;6(9):FSO612.
doi: 10.2144/fsoa-2020-0023.

Nontuberculous mycobacteria - clinical and laboratory diagnosis: experiences from a TB endemic country

Ann Susan Sam  1 , Marilyn Mary Ninan  1 , R Ranjani  1 , Naveen Kumar Devanga Raghupathi  1 , Veeraraghavan Balaji  1 , Joy Sarojini Michael  1
Affiliations

Nontuberculous mycobacteria - clinical and laboratory diagnosis: experiences from a TB endemic country

Ann Susan Sam et al. Future Sci OA. .

Abstract

Aim: To evaluate the performance of VITEK®MS with DNA sequencing for laboratory diagnosis of non-tuberculous mycobacteria (NTM) species in a resource-limited setting.

Methods: 16SrRNA sequencing and MALDI-TOF mass spectrometry (VITEK®MS) was performed at a tertiary-care hospital in India. MALDI-TOF results were confirmed by 16S rRNA sequencing. In addition, sequencing of the internal transcribed spacer region was performed on slowly growing NTM.

Results: Commonest species isolated were M. abscessus, M. intracellulare, M. avium, M. fortuitum and M. simiae. 16S rRNA sequencing and MALDI-TOF results had agreement of 94.5% for rapidly growing and 77.5% for slowly growing NTM.

Conclusion: There is good correlation between VITEK®MS and sequencing for rapidly growing NTM. For slowly growing species, sequencing would be required in a third isolates.

Keywords: MALDI-TOF mass spectrometry; VITEK®MS; geographic distribution; identification; nontuberculous mycobacteria; prevalence; sequencing.

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Conflict of interest statement

Financial & competing interests disclosure This work was supported by the FLUID research grant of Christian Medical College Vellore (Institutional Review Board min no. 11224). The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

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