doi: 10.1016/0076-6879(87)54076-9.
5-Fluoroorotic acid as a selective agent in yeast molecular genetics
- PMID: 3323810
- DOI: 10.1016/0076-6879(87)54076-9
Item in Clipboard
5-Fluoroorotic acid as a selective agent in yeast molecular genetics
Methods Enzymol.
1987.
Abstract
5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.
Similar articles
-
New shuttle vectors for direct cloning in Saccharomyces cerevisiae.Gene. 1991 Jul 31;104(1):99-102. doi: 10.1016/0378-1119(91)90472-n. Gene. 1991. PMID: 1916284
-
Identification of the orotidine-5'-phosphate decarboxylase gene and development of a transformation system in the yeast Saccharomyces exiguus Yp74L-3.Biosci Biotechnol Biochem. 1998 Nov;62(11):2280-2. doi: 10.1271/bbb.62.2280. Biosci Biotechnol Biochem. 1998. PMID: 9972254
-
Transformation of the yeast Saccharomyces kluyveri by Saccharomyces cerevisiae-based plasmids.FEMS Microbiol Lett. 1991 Aug 1;66(2):149-52. doi: 10.1016/0378-1097(91)90324-4. FEMS Microbiol Lett. 1991. PMID: 1936943
-
Yeast vectors for integration at the HO locus.Nucleic Acids Res. 2001 Jun 15;29(12):E59-9. doi: 10.1093/nar/29.12.e59. Nucleic Acids Res. 2001. PMID: 11410682 Free PMC article.
-
Salinity tolerance--mechanisms, models and the metabolic engineering of complex traits.Genet Eng (N Y). 1998;20:153-76. doi: 10.1007/978-1-4899-1739-3_9. Genet Eng (N Y). 1998. PMID: 9666560 Review. No abstract available.
Cited by
-
Identification and functional characterization of novel xylose transporters from the cell factories Aspergillus niger and Trichoderma reesei.Biotechnol Biofuels. 2016 Jul 20;9:148. doi: 10.1186/s13068-016-0564-4. eCollection 2016. Biotechnol Biofuels. 2016. PMID: 27446237 Free PMC article.
-
Synthetic Platforms for Characterizing and Targeting of SARS-CoV-2 Genome Capping Enzymes.ACS Synth Biol. 2022 Nov 18;11(11):3759-3771. doi: 10.1021/acssynbio.2c00359. Epub 2022 Nov 4. ACS Synth Biol. 2022. PMID: 36331143 Free PMC article.
-
Relationships Between RNA Polymerase II Activity and Spt Elongation Factors to Spt- Phenotype and Growth in Saccharomyces cerevisiae.G3 (Bethesda). 2016 Aug 9;6(8):2489-504. doi: 10.1534/g3.116.030346. G3 (Bethesda). 2016. PMID: 27261007 Free PMC article.
-
Sites of acetylation on newly synthesized histone H4 are required for chromatin assembly and DNA damage response signaling.Mol Cell Biol. 2013 Aug;33(16):3286-98. doi: 10.1128/MCB.00460-13. Epub 2013 Jun 17. Mol Cell Biol. 2013. PMID: 23775118 Free PMC article.
-
Functional characterization of piggyBat from the bat Myotis lucifugus unveils an active mammalian DNA transposon.Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):234-9. doi: 10.1073/pnas.1217548110. Epub 2012 Dec 17. Proc Natl Acad Sci U S A. 2013. PMID: 23248290 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
