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. 1987:154:164-75.
doi: 10.1016/0076-6879(87)54076-9.

5-Fluoroorotic acid as a selective agent in yeast molecular genetics

J D Boeke, J Trueheart, G Natsoulis, G R Fink

5-Fluoroorotic acid as a selective agent in yeast molecular genetics

J D Boeke et al. Methods Enzymol. 1987.

Abstract

5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.

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