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. 1987 Nov;7(11):4146-9.
doi: 10.1128/mcb.7.11.4146-4149.1987.

Involvement of functional protein kinase C in the mitogenic response to the H-ras oncogene product

J C Lacal  1 , T P Fleming, B S Warren, P M Blumberg, S A Aaronson
Affiliations

Involvement of functional protein kinase C in the mitogenic response to the H-ras oncogene product

J C Lacal et al. Mol Cell Biol. 1987 Nov.

Abstract

Microinjection of purified protein kinase C (PKC) into Swiss 3T3 fibroblasts pretreated with the phorbol ester phorbol-12,13-dibutyrate restores the mitogenic response of the cells to phorbol-12,13-dibutyrate (G. Pasti, J.C. Lacal, B.S. Warren, S.A. Aaronson, and P.M. Blumberg, Nature [London] 324:375-377, 1986). Our present studies demonstrate that the mitogenic activity of the H-ras oncogene in H-ras p21-microinjected quiescent cells is markedly reduced under conditions in which PKC is downregulated by chronic phorbol ester treatment. The ability to reconstitute the mitogenic response upon microinjection of both H-ras p21 and PKC implies involvement of functional PKC in the mitogenic activity of the H-ras oncogene product.

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References

    1. J Cell Physiol. 1982 Jul;112(1):42-50 - PubMed
    1. J Cell Physiol. 1984 Feb;118(2):133-42 - PubMed
    1. Hum Genet. 1984;66(2-3):132-42 - PubMed
    1. Biochem Biophys Res Commun. 1984 May 16;120(3):1053-9 - PubMed
    1. Nature. 1984 Aug 9-15;310(5977):508-11 - PubMed