Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Oct:(10):19-23.

[Expression in Escherichia coli cells of the nucleotide sequence of DNA coding for the bovine lipotropic hormone]

[Article in Russian]
A B Beklemishev, S Ia Golovin, A A Il'ichev, L V Mamaev, V N Krasnykh
  • PMID: 3323897

[Expression in Escherichia coli cells of the nucleotide sequence of DNA coding for the bovine lipotropic hormone]

[Article in Russian]
A B Beklemishev et al. Mol Gen Mikrobiol Virusol. 1987 Oct.

Abstract

A cDNA fragment of bovine proopiomelanocortin coding for beta-lipotropic hormone was joined with a promoter and ribosome binding site of B. amyloliquefaciens and cloned in E. coli in pBR 327 plasmid. The level of beta-lipotropin synthesis in bacterial cells transformed by the obtained plasmid was estimated immunochemically. The level of beta-lipotropin production was shown to be 5 mg per liter of bacterial culture.

PubMed Disclaimer