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. 2021 Mar;68(2):960-963.
doi: 10.1111/tbed.13777. Epub 2020 Sep 11.

A genetic variant of Burkholderia mallei detected in Kuwait: Consequences for the PCR diagnosis of glanders

Karine Laroucau  1 , Rachid Aaziz  1 , Fabien Vorimore  1 , Koshy Varghese  2 , Thomas Deshayes  1 , Claire Bertin  1 , Sabine Delannoy  3 , Attia M Sami  2 , Maha Al Batel  2 , Mamdouh El Shorbagy  2 , Khaled A W Almutawaa  2 , Saad J Alanezi  2 , Yousef S N Alazemi  2 , Vanina Guernier-Cambert  4 , Ulrich Wernery  5
Affiliations

A genetic variant of Burkholderia mallei detected in Kuwait: Consequences for the PCR diagnosis of glanders

Karine Laroucau et al. Transbound Emerg Dis. 2021 Mar.

Abstract

Glanders is a contagious zoonotic disease caused by Burkholderia mallei. Following the detection of glanders positive horses using the OIE complement fixation test, the tissues of two horses were analysed by PCR. While PCR systems targeting the Burkholderia pseudomallei complex gave positive signals, the species-specific PCR systems targeting B. mallei (fliP-IS407A) and B. pseudomallei (orf11)-the OIE recommended targets-resulted in negative signals. However, the presence of B. mallei in these tissues was confirmed with a recently described B. mallei-specific real-time PCR system and genotyping with MLST- and SNP-based methods, performed on the most positive tissue, identified a genotype closely related to B. mallei strains recently isolated in the Middle East. This study leads to recommendations regarding the use of PCR systems for the molecular diagnosis of glanders, especially in regions where the circulating B. mallei strains have not yet been fully genetically characterized.

Keywords: Burkholderia mallei; fliP; PCR diagnosis; glanders; insertion sequence; variant.

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