Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Nov 1;21(11):3373-3379.
doi: 10.31557/APJCP.2020.21.11.3373.

Assessment of Tumor Angiogenesis by Expression of CD 105 in Ameloblastoma, Odontogenic Keratocyst and Central Giant Cell Lesion

Khurshid Ali  1 , Sultan Zeb Khan  1 , Nuzhat Sultana  2 , Osama Alghamdi  3 , Samrina Muhammad  1 , Sameer A Mokeem  4 , Saqib Ali  5 , Tariq Abduljabbar  6 , Fahim Vohra  6
Affiliations

Assessment of Tumor Angiogenesis by Expression of CD 105 in Ameloblastoma, Odontogenic Keratocyst and Central Giant Cell Lesion

Khurshid Ali et al. Asian Pac J Cancer Prev. .

Abstract

Background: Angiogenesis is critical for tumor growth and reflects the aggressive behavior of invasive odontogenic lesions [like Ameloblastoma (AM), Odontogenic Keratocyst (OKC) and Central giant cell lesion (CGCL)]. Mean vascular density (MVD) shows the angiogenic potential and CD105 is an ideal endothelial biomarker due to its specificity to new blood vessels for MVD detection. The aim of the study was to compare the MVD (angiogenic potential) among AM, OKC and CGCL in comparison to Pyogenic Granuloma (PG) using CD105 biomarker.

Methods: Sixty-four primary cases of odontogenic invasive tumors (AM, OKC and CGCL) and PG, diagnosed clinically and histologically were included in the study, with 16 samples in each group. Tissue samples of peripheral AM, Peripheral GCL of jaws, malignant AM, and specimen with insufficient tissue were excluded. Tissue sections were embedded, processed and stained using Hematoxylin and Eosin (H and E). Immunohistochemistry was performed using antibodies against CD105, with positive brown cytoplasmic staining in the endothelial cells of neo-vasculature. Distinct countable, positively stained endothelial cell or clusters were evaluated under light microscope for identification of MVD. ANOVA and t-test were applied for statistical analysis of data.

Results: Highest MVD was displayed in CGCL (32.99±0.77) and the minimum was observed in OKC (7.21± 0.75) respectively. CGCL showed significantly higher MVD to AM, OKC and PG lesions (p <0.05). AM (8.07± 0.36) and Odontogenic Keratocyst (7.21± 0.75) showed comparable MVD, which was lower than PG (14.7± 0.96) and CGCL vascular density (p < 0.01) respectively.

Conclusion: CGCL was most aggressive, with highest MVD among the investigated odontogenic lesions (OKC, AM and PG). The proliferative aggressive behavior of Odontogenic Keratocyst is comparable to AM due to comparable mean vascular density.<br />.

Keywords: Blood vessels; Endoglin; Giant cell granuloma; Pathologic angiogenesis, Odontogenic tumors.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Photomicrograph of H & E Staining (A) and Immunohistochemical staining (B) with CD105, of micro vessels around giant cells in a Central giant cell lesion section
Figure 2
Figure 2
Photomicrograph of H & E Staining (A) and Immunohistochemical staining (B) with CD105, of micro vessels in a Pyogenic granuloma section
Figure 3
Figure 3
Photomicrograph of H & E Staining (A) and Immunohistochemical staining (B) with CD105, of micro vessels in connective tissue wall beneath epithelium in a Odontogenic Keratocyst section
Figure 4
Figure 4
Photomicrograph of H & E Staining (A) and Immunohistochemical staining (B) with CD105, of micro vessels adjacent to epithelial islands in an AM section
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Alaeddini M, Salah S, Dehghan F, Eshghyar N, Etemad Moghadam S. Comparison of angiogenesis in keratocystic odontogenic tumours, dentigerous cysts and AMs. Oral Dis. 2009;15:422–7. - PubMed
    1. ALI MAA. Expression of extracellular matrix metalloproteinase inducer in odontogenic cysts. Oral Surg Oral Med Oral Pathol Oral Radiol Endodontol. 2008;106:258–63. - PubMed
    1. Amaral FCR, Gomes CCR, Oliveira CS, Souza PEAD, Gomez , RS Clonality analysis of giant cell lesions of the jaws. Brazil Dent J. 2010;21:361–4. - PubMed
    1. Bosari S, Lee AK, Delellis RA, et al. Microvessel quantitation and prognosis in invasive breast carcinoma. Hum Pathol. 1992;23:755–61. - PubMed
    1. Chang CS, Hsiao YC, Huang F, et al. Giant cell reparative granuloma of the mandibular condyle: a long-term case report and review of the literature. Plastic Surg Case Studies. 2016;2:44–6.

MeSH terms