Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Dec;99(12):6576-6585.
doi: 10.1016/j.psj.2020.09.075. Epub 2020 Oct 8.

Yeast cell wall product enhanced intestinal IgA response and changed cecum microflora species after oral vaccination in chickens

S Bi  1 , J Zhang  1 , Y Qu  1 , B Zhou  1 , X He  1 , J Ni  2
Affiliations

Yeast cell wall product enhanced intestinal IgA response and changed cecum microflora species after oral vaccination in chickens

S Bi et al. Poult Sci. 2020 Dec.

Abstract

The study was designed to explore the effect of a commercial yeast cell wall product (YP) on chicken intestinal IgA response and cecum microbiome after oral vaccination. Chickens were fed with YP during the experiments and orally immunized with live Newcastle disease virus (NDV) vaccine at 2 wk of age. Then, the animals were sacrificed, and samples were collected to measure the indicators of hemagglutination inhibition (HI), IgA response, IgA + cells, and cecum microbiome populations. The results showed that supplement of YP significantly enhanced serum NDV HI titer, intestinal NDV-specific secretory IgA, and intestinal IgA + cells. The sequencing results revealed that obviously increased relative abundance of Ruminococcaceae and decreased population of Bacteroidaceae in cecum were found in YP group. In summary, YP supplementation in diet enhanced intestinal IgA response to NDV vaccination by oral route and modulated the cecum microbiota to the advantage of the host in chickens.

Keywords: Newcastle disease virus vaccine; cecum microflora; intestinal IgA response; yeast cell wall product.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Antibody responses to vaccine of Newcastle disease virus (NDV) were assessed by HI titers. (A) Chickens (n = 15/group) were feeds with 0, 0.05, 0.1, or 0.2% YP and received oral immunization with a live NDV vaccine. Blood samples were collected 1, 2 wk postimmunization for analysis of NDV-specific HI titers. (B) Chickens (n = 15/group) were feeds with 0 or 0.1% YP and received oral immunization with a live NDV vaccine. Blood samples were collected before and 1, 2, 3, and 4 wk postimmunization for analysis of NDV-specific HI titers. The data were expressed as the log 2 of the highest dilution that exhibited complete inhibition of hemagglutination. The values were represented as mean ± SE. The red line represents the minimum positive antibody titer. Abbreviation: HI, hemagglutination inhibition.
Figure 2
Figure 2
NDV-specific IgA in feces washing fluid was detected by ELISA. Chickens were fed with supplement of YP (0 or 0.1%) and received oral immunization with a live vaccine of NDV. Feces were collected before and 1, 2, 3, and 4 wk postimmunization for analysis of NDV-specific sIgA antibody. The values were represented as mean ± SE (n = 15). Abbreviations: NDV, Newcastle disease virus; YP, yeast cell wall product.
Figure 3
Figure 3
The number of IgA + cells. Chickens were fed with supplement of YP (0 or 0.1%) and received oral immunization with a live vaccine of NDV. The duodenum tissue samples were collected and fixed in 10% neutral buffered formalin (v/v) for immunohistochemical staining (400×). (A) IgA + cells of distributed in the lamina propria of the villi in the duodenum. Arrowheads represent positive cells. Scale bars: 50 μm. (B) Quantification of IgA + cell number at 10 different fields. The values were represented as mean ± SE (n = 10). Abbreviations: NDV, Newcastle disease virus; YP, yeast cell wall product.
Figure 4
Figure 4
Alpha diversity between groups. (A) Average observed species and (B) average Chao1; CONTROL: normal feed and immunized with NDV vaccine, YP: normal feed with 0.1% YP and immunized with NDV vaccine. Abbreviations: NDV, Newcastle disease virus; YP, yeast cell wall product.
Figure 5
Figure 5
Beta diversity analysis between groups. Weighted PCoA plots of individual birds in each group. Individual sample was represented as spot with red (CONTROL: normal feed and immunized with NDV vaccine) and blue (YP; normal feed with 0.1% YP and immunized with NDV vaccine). Abbreviations: NDV, Newcastle disease virus; YP, yeast cell wall product.
Figure 6
Figure 6
Classification of cecum flora compositions with (A) phylum level or (B) family level. CONTROL: normal feed and immunized with NDV vaccine, YP: normal feed with 0.1% YP and immunized with NDV vaccine. Abbreviations: NDV, Newcastle disease virus; YP, yeast cell wall product.
Figure 7
Figure 7
Comparison of cecum bacteria among groups in (A) phylum level or (B) family level. CONTROL: normal feed and immunized with NDV vaccine, YP: normal feed with 0.1% YP and immunized with NDV vaccine. The values were represented as mean ± SE (n = 6). Abbreviations: NDV, Newcastle disease virus; YP, yeast cell wall product.
See this image and copyright information in PMC

References

    1. Alizadeh M., Rodriguez-Lecompte J.C., Rogiewicz A., Patterson R., Slominski B.A. Effect of yeast-derived products and distillers dried grains with solubles (DDGS) on growth performance, gut morphology, and gene expression of pattern recognition receptors and cytokines in broiler chickens. Poult. Sci. 2016;95:507–517. - PubMed
    1. Beirao B., Ingberman M., Favaro C.J., Mesa D., Bittencourt L.C., Fascina V.B., Caron L.F. Effect of an Enterococcus faecium probiotic on specific IgA following live Salmonella enteritidis vaccination of layer chickens. Avian Pathol. 2018;47:325–333. - PubMed
    1. Berndt A., Wilhelm A., Jugert C., Pieper J., Sachse K., Methner U. Chicken cecum immune response to Salmonella enterica serovars of different levels of invasiveness. Infect. Immun. 2007;75:5993–6007. - PMC - PubMed
    1. Brisbin J.T., Gong J., Orouji S., Esufali J., Mallick A.I., Parvizi P., Shewen P.E., Sharif S. Oral treatment of chickens with Lactobacilli influences elicitation of immune responses. Clin. Vaccine Immunol. 2011;18:1447–1455. - PMC - PubMed
    1. Chao X.P., Sun T.T., Wang S., Fan Q.B., Shi H.H., Zhu L., Lang J.H. Correlation between the diversity of vaginal microbiota and the risk of high-risk human papillomavirus infection. Int. J. Gynecol. Cancer. 2019;29:28–34. - PubMed