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. 2020 Oct 28:38:100801.
doi: 10.1016/j.nmni.2020.100801. eCollection 2020 Nov.

Draft genome and description of Corynebacterium haemomassiliense strain Marseille-Q3615T sp. nov., a new bacterium isolated from a 59-year-old man with chronic obstructive pulmonary disease symptoms

M Boxberger  1   2 , A Antezack  1   2 , S Magnien  1   2 , N Cassir  1   2 , B La Scola  1   2
Affiliations

Draft genome and description of Corynebacterium haemomassiliense strain Marseille-Q3615T sp. nov., a new bacterium isolated from a 59-year-old man with chronic obstructive pulmonary disease symptoms

M Boxberger et al. New Microbes New Infect. .

Abstract

In 2020, as part of a diagnosis attempt at IHU Méditerranée Infection in Marseille (France), a blood specimen was obtained from a 59-year-old man with chronic obstructive pulmonary disease symptoms, from which we isolated the new bacterial Corynebacterium haemomassiliense strain Marseille-Q3615T. Matrix-assisted desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) failed to identify this isolate. Analysis of the 16S ribosomal RNA gene and genome-to-Genome comparison suggested that this taxon belongs to a novel bacterial species within the family Corynebacteriaceae in the phylum Actinobacteria. We describe the main phenotypic characteristics, genome sequence and annotation of Corynebacterium haemomassiliense strain Marseille-Q3615T, a new member of the Corynebacterium genus, which we propose as the type strain.

Keywords: Bacteria; corynebacterium; genome; human; sp. nov.; species; taxonogenomics.

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Figures

Fig. 1
Fig. 1
MALDI-TOF MS reference mass spectrum. Spectra from 12 individual colonies of Corynebacterium haemomassiliense strain Marseille-Q3615T were compared and reference spectrum generated.
Fig. 2
Fig. 2
16S rRNA gene (A) and rpoB gene (B) based phylogenetic trees highlighting position of Corynebacterium haemomassiliense sp. nov. strain Marseille-Q3615 (red) relative to other closely related bacterial taxa. Sequences were aligned by Muscle v3.8.31 with default parameters; phylogenetic relationship was inferred by maximum likelihood method, with 1000 bootstrap replicates, within MEGA 7 software.
Fig. 3
Fig. 3
Scanning electron micrograph of Corynebacterium haemomassiliense sp. nov. strain Marseille-Q3615T using TM4000Plus tabletop microscope (Hitachi High-Tech, Tokyo, Japan). Scale bar represents 5 μm.
Fig. 4
Fig. 4
Graphical circular map of genome from strain Marseille-Q3615T obtained by CGView Server tool [14].
Fig. 5
Fig. 5
Heat map generated with OrthoANI values calculated by OAT software between Corynebacterium haemomassiliense sp. nov. strain Marseille-Q3615T and other closely related species with standing in nomenclature.
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