[The experimental study on the biological rhythm expression of substance P in allergic airway inflammation mice]

Abstract

Objective:To establish the murine models of allergic airway inflammation, and to investigate the biological rhythm expression of substance P in it. Method:Eighty-four BALB/c mice were divided into 14 groups, which were airway inflammation groups（0: 00 group, 04: 00 group, 08: 00 group, 12: 00 group, 16: 00 group, 20: 00 group, 24: 00 group） and normal control groups（0: 00 group, 04: 00 group, 08: 00 group, 12: 00 group, 16: 00 group, 20: 00 group, 24: 00 group）. The allergic airway inflammation models of mice were established by intraperitoneal injection of ovalbumin（OVA）, nasal challenge and aerosol inhalation with OVA. The plasma substance P levels were measured by ELISA. The expression of substance P in nasal mucosa, left lung tissue and left bronchial tissue during different time periods was obversed with RT-PCR and immunohistochemical technique. Result:In allergic airway inflammation groups, the plasma substance P levels were higher than in normal control groups（P<0.05）, and 0: 00 group（321.99±28.93） pg/mL, 04: 00 group（310.76±25.31） pg/mL and 24: 00 group（335.62±28.29） pg/mL were higher than other experimental groups（P<0.05）. The expression of substance P mRNA in nasal mucosa, left lung tissue and left bronchial tissue was higher than control groups（P<0.01）. By observing immunohistochemical sections, the expression of substance P in allergic airway inflammation groups was higer than control groups. Conclusion:The expression of substance P is high in allergic airway inflammation mice and the peaks are during 04: 00-08: 00 in the early morning, which may provide new basis for treating allergic airway inflammation.

Keywords: allergic airway inflammation; biological rhythm; substance P.

图 1

造模周期图

图 2

血液与组织中P物质表达趋势

图 3

病理形态学观察