The plasma lipidome of the Quaker parrot (Myiopsitta monachus)

Abstract

Dyslipidemias and lipid-accumulation disorders are common in captive parrots, in particular in Quaker parrots. Currently available diagnostic tests only measure a fraction of blood lipids and have overall problematic cross-species applicability. Comprehensively analyzing lipids in the plasma of parrots is the first step to better understand their lipid metabolism in health and disease, as well as to explore new lipid biomarkers. The plasma lipidome of 12 Quaker parrots was investigated using UHPLC-MS/MS with both targeted and untargeted methods. Targeted methods on 6 replicates measured 432 lipids comprised of sterol, cholesterol ester, bile acid, fatty acid, acylcarnitine, glycerolipid, glycerophospholipid, and sphingolipid panels. For untargeted lipidomics, precursor ion mass-to-charge ratios were matched to corresponding lipids using the LIPIDMAPS structure database and LipidBlast at the sum composition or acyl species level of information. Sterol lipids and glycerophospholipids constituted the majority of plasma lipids on a molar basis. The most common lipids detected with the targeted methods included free cholesterol, CE(18:2), CE(20:4) for sterol lipids; PC(36:2), PC(34:2), PC(34:1) for glycerophospholipids; TG(52:3), TG(54:4), TG(54:5), TG(52:2) for glycerolipids; SM(d18:1/16:0) for sphingolipids; and palmitic acid for fatty acyls. Over a thousand different lipid species were detected by untargeted lipidomics. Sex differences in the plasma lipidome were observed using heatmaps, principal component analysis, and discriminant analysis. This report presents the first comprehensive database of plasma lipid species in psittacine birds and paves the way for further research into blood lipid diagnostics and the impact of diet, diseases, and drugs on the parrot plasma lipidome.

Fig 1. Circular barplot of the mean concentration of lipid species across 5 lipid categories measured by targeted lipidomics in Quaker parrots (Myiopsitta monachus).

The dashed circle and arrow indicate the zoomed-in portion of the left barplot. Each lipid category is color coded differently. Lipid species abbreviation follows the LIPIDMAPS nomenclature. While lipid species were quantitively measured, comparisons across lipid category should be made carefully.

Fig 2. PCA scores plot (A) and PLS-DA scores plot (B) between the first two components showing clustering of male and female Quaker parrots (Myiopsitta monachus) using targeted lipidomics panel between sexes.

Grouping is shown as different colors with their 95% confidence ellipses. The explained variances are shown in brackets.

Fig 3. Heatmap showing clustering of lipid species from targeted lipidomics panels between sexes in Quaker parrots (Myiopsitta monachus).

A clustering dendrogram is also present on the left, the different parrots are on the x-axis and the lipid analytes on the y-axis. Only the 50 most important lipids based on their t-test p-values are displayed. It should be noted that most of these lipids did not show significant differences between sexes on univariate analysis. Color coding represents fold changes on normalized plasma concentrations.