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Case Reports
. 2020 Nov 12;106(6):730-734.
doi: 10.1645/19-196.

A Case-Study of the Molecular Diagnosis of Echinococcus multilocularis in Wild Boar with Comments on its Public Health Significance in Turkey

Harun Kaya Kesik  1 , Seyma Gunyakti Kilinc  1   2 , Figen Celik  2 , Sami Simsek  2 , Haroon Ahmed  3
Affiliations
Case Reports

A Case-Study of the Molecular Diagnosis of Echinococcus multilocularis in Wild Boar with Comments on its Public Health Significance in Turkey

Harun Kaya Kesik et al. J Parasitol. .

Abstract

Echinococcus multilocularis is a parasite species of zoonotic importance which can be fatal to humans and causes Alveolar Echinococcosis (AE). This report describes the development of a cyst from the liver of a wild boar and molecular confirmation of its identification. The cyst material was obtained from the liver of a wild boar killed by hunters. Genomic DNA was extracted from the germinal layer of the cyst material, and 875 bp mitochondrial cytochrome c oxidase subunit I (COI) gene fragment was amplified by PCR and sequenced. A BLAST search matched 100% with published Echinococcus multilocularis sequences. This study confirms the occurrence of E. multilocularis in a wild boar for the first time in Turkey.

Keywords: Echinococcus multilocularis; PCR; Sequencing; Turkey; Wild Boar.

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